Ploidisasi Ikan Mystacoleucus padangensis Bleeker, 1852 (Pisces: Cyprinidae) Melalui Induksi Kejutan Panas (Heat Shock)

Authors

  • Dian Juliadmi
  • Dewi Imelda Roesma
  • Djong Hon Tjong

DOI:

https://doi.org/10.25077/jbioua.4.1.%25p.2015

Abstract

Ploidization of Mystacoleucus padangensis Bleeker, 1852 (Pisces: Cyprinid) with heat shock induction has been carried out from January to September 2014 at Genetics and Cytology Laboratory, Biology Department of Faculty of Mathematics and Natural Sciences, Andalas University. This study aimed to find suitable temperature for ploidizatiom on M. padangensis. The study used Completely Randomized Design method with five treatments and five replications. The treatments were various water temperatures (i.e. 27oC as control, 36oC, 38oC, 40oC, and 42oC). The results showed that triploid individuals were found in all treatments but not control.

 

Keywords: ploidization, Mystacoleucus padangensis, heat shock

References

Azhar. 1993. Studi Ekologi Ikan Bilih (Mystacoleucus padangensis Blkr.) di Danau Singkarak, Sumatera Barat. Tesis. Program Pasca Sarjana IPB Bogor: 134 hlm.

Bencsik, I., N. Pacala, G. Dumitrescu, D. Dronca, J. Stanculet, L. P. Ciochina, and L. Boca. 2011. The Rate of Tetraploidy Determination in Rainbow Trout (Oncorhynchus mykiss) in Embryonated Eggs. Scientific Papers: Animal Science and Biotechnologies. 44 (1): 158-161.

Berrebi, P. 1995. Speciation of the genus Barbus in The North Mediterranean basin: Recent advances from biochemical genetics. Biological Conservation. 72: 237-249.

Carman, O., T. Oshiro, and F. Takashima. 1992. Variation in The Maximum Number of Nucleoli in Diploid and Triploid Commom Carp. Nippon Suisan Gakkaishi, 58.

Collares-Pereira, M. J. 1989. Hybridization in European Cyprinids: Evolutionary Potential of Unisexual Populations (In: R. M. Dawley ed.) Evolution and Ecology of Unisexual Vertebrates. Bulletin: New York.

Direktorat Jendral Perikanan Tangkap. 2005. Upaya Pelestarian Ikan Bilih di Danau Singkarak. Kementerian Kelautan dan Perikanan.

Direktorat Jendral Perikanan Tangkap. 2012. Laporan Akuntabilitas Kinerja Instansi Pemerintah. Kementerian Kelautan dan Perikanan.

Direktorat Perlindungan dan Pengawetan Alam (DPPA). 1980. Pedoman Pengelolaan Satwa Langka, Jilid III Serangga, Ikan serta Reptilia dan Mamalia Laut. Direktorat Jendral Kehutanan: Bogor.

Haryanto, A. 2004. Pengaruh Lama Waktu Kejutan Suhu Terhadap Tingkat Keberhasilan Ginogenesis pada Ikan Sumatera (Puntius tetrazona). Skripsi. Institut Pertanian Bogor.

Herbst, E.C. 2002. Induction Of Tetraploidy In Zebrafish Danio Rerio and Nile Tilapia Oreochromis Niloticus. Thesis. University of North Carolina at Charlotte. 139 hal.

Hollebecq, M. G., D. Chourrout, G. Whohifarth, and R. Billard. 1986. Diploid Gynogenesis Induced by Heat Shock After Activation with UV-Irradiated sperm in Common Carp. Aquaqulture, 54: 69-76. (Abstract)

Junaidi, E., E. Patriono, and F. Sastra. 2009. Indeks Gonad Somatik Ikan Bilih (Mystacoleucus padangensis Blkr.) yang Masuk ke Muara Sungai Sekitar Danau Singkarak. Jurnal Penelitian Sains 09:12-12.

Kartamihardja, E.S. and A.S. Sarnita. 2008. Populasi Ikan Bilih di Danau Toba: Keberhasilan Introduksi Ikan Implikasi Pengelolaaan dan Prospek Masa Depan. Pusat Riset Perikanan Tangkap. Badan Riset Kelautan dan Perikanan. Departemen Kelautan Dan Perikanan.

Kilic-Demirok, N. and Unlu, E. 2004. Karyotype of The Cyprinid Fish Alburnoides bipunctatus (Cyprinidae) from The Tigris River. Folia Biologica (Krakow) 52: 57-59.

Kirtiklis L., A. Boron, and K. Porycka. 2005. Chromosome banding patterns of the gudgeon, Gobio gobio (Actinopterygii, Cyprinidae). Acta Ichthyologica et Piscatoria. 35(2): 119–123.

Kottelat, M. A., J. Whitten, S. N. Kartikasari, and S. Wirjoatmodjo. 1993. Freshwater Fishes of Western Indonesia and Sulawesi. Barkeley Books. Pte Itd, Terrer Road: Singapore.

Mukti, A.T. 2005. Perbedaan Keberhasilan Tingkat Poliploidisasi Ikan Mas (Cyprinus Carpio Linn.) Melalui Kejutan Panas. J.Berk. Penel. Hayati, 10: 133–138.

Nurasni, A. 2012. Pengaruh Suhu dan Lama Kejutan Panas terhadap Triploidisasi Ikan Lele Sangkuriang (Clarias gariepinus). IJAS 2(1): 19-23.

Pradeep, P.J., T.C. Srijaya, R.B.M. Zaini, A. Papini and A.K. Chatterji. 2011. A Simple Technique for Chromosome Preparation from Embryonic Tissues of teleosts for Ploidy Verifi cation. Caryologia, 64(2): 235-241.

Rahmawati, I. 2006. Studi Reproduksi Ikan Beunteur (Puntius binotatus, C.V. 1842, Famili Cyprinidae) di Bagian Hulu Daerah Aliran Sungai (DAS) Ciliwung, Jawa Barat. Skripsi. Institut Pertanian Bogor.Tidak dipublikasikan.

Roesma, D.I. 2011. Diversitas Spesies dan Kekerabatan Genetik Ikan-Ikan Cyprinidae di Danau-Danau dan Sungai-Sungai di Sekitarnya di Kawasan Sumatera Barat. Disertasi. Program Pasca Sarjana Universitas Andalas. 199 hal.

Roesma, D.I., Syaifullah, and Melyawati. 2012. Pengamatan Kromosom Ikan Bilih (Mystacoleucus padangensis BLKR., Cyprinidae) dari Danau Singkarak Sumatera Barat. Biospecies. 5(2):1-4.

Sundov, Z., Z. Nincevicb, M. Definis-Gojanovicc, M. Glavina-Durdovc, I. Jukica, N. Hulinand, and A. Tonkica. 2005. Fatal Colchinine Poisining by Accidental Ingestion of Meadow Saffron-Case Report. Forensic Sciences International 149: 253-256.

Syandri, H. 1990. Beberapa Aspek Biologi Ikan Bilih (Mystacoleucus padangensis Blkr) di Perariran Umum Danau Singkarak Sumatera Barat. Laporan Penelitian. Universitas Bung Hatta.

Syandri, H.1996. Aspek reproduksi ikan bilih, Mystacoleucus padangensis Bleeker dan kemungkinan pembenihannya di Danau Singkarak. Disertasi. Program Pascasarjana IPB. 122 hal.

Syandri, H. 1997. Perkembangan Oosit dan Testis Ikan Bilih (Mystacoleucus padangensis Blkr) di Danau Singkarak Sumatera Barat. Fish J. Garing 2: 8-20.

Syandri, H. 2008. Ancaman terhadap Plasma Nutfah Ikan Bilih (Mystacoleucus padangensis Blkr) dan Upaya Pelestariannya di Danau Singkarak. Laporan Penelitian. Fakultas Perikanan dan Ilmu Kelautan Universitas Bung Hatta.

Yamada K., Ichikawa Y., Okumura H. 1989: Effects of High Temperatures on Chromosomes of Normal and Transformed Human Cell. Human Cell, 2: 80–85.

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Published

2015-03-01

How to Cite

Juliadmi, D., Roesma, D. I., & Tjong, D. H. (2015). Ploidisasi Ikan Mystacoleucus padangensis Bleeker, 1852 (Pisces: Cyprinidae) Melalui Induksi Kejutan Panas (Heat Shock). Jurnal Biologi UNAND, 4(1). https://doi.org/10.25077/jbioua.4.1.%p.2015

Issue

Vol. 4 No. 1 (2015)

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