Optimasi Parsial Isolat Termofilik M5-24 dalam Produksi Protease

Authors

  • Aghni Aznia Laboratorium Riset Mikrobiologi, Jurusan Biologi, FMIPA Universitas Andalas, Kampus UNAND Limau Manis Padang -25163
  • Anthoni Agustien Laboratorium Riset Mikrobiologi, Jurusan Biologi, FMIPA Universitas Andalas, Kampus UNAND Limau Manis Padang -25163
  • Nasril Nasir Laboratorium Riset Mikrobiologi, Jurusan Biologi, FMIPA Universitas Andalas, Kampus UNAND Limau Manis Padang -25163

DOI:

https://doi.org/10.25077/jbioua.3.3.%25p.2014

Abstract

Research about partial optimization of thermophilic bacteria isolate M5-24 on protease production was conducted from May 2013 to December 2013 in Laboratory of Microbiology, Biology Department, Andalas University, Padang. Purpose of the research was to find out optimum condition of protease production by isolate M5-24. This study consisted of three steps. Step 1: Investigating the effect of temperature and pH used completely randomized design in factorial with two replications.The first factor was temperature (450C, 500C, 550C, 600C), the second factor was pH (7, 8, 9, 10). Step 2: Investigating the effect of inoculum doses and inducers used completely randomized design in factorial with three replications. The first factor was inoculum doses (1%, 5%, 10%) and the second factor was inducer (yeast extract 0,5%; pepton 0,1%;  kasein 1%; meat extract 0,5%). While Step 3: Investigating the effect of agitation (125 rpm, 150 rpm, 175 rpm, 200 rpm) used completely randomized design with six replications. The result showed that the best duration of growing was at 18th hours after incubation, the highest enzyme activity was at temperature 500 C;  medium’s pH at 8; the best of inoculum dose was at 5%; the best  inducer was casein 1% and optimum agitation was at 175 rpm. Extrinsic optimization can increase protease production of  thermophilic isolate M5-24 up to 1.7 times.

Keywords : Optimization, Protease,  thermophilic, bacteria

References

Agustien, A. 2010. Protease Bakteri Termofilik. Universitas Padjajaran Press. Bandung

Ahmed, I., M. Irfan, M. Nadeem, M.A. Zia, B.M. Ahmad, M. Hafiz and N. Iqbal. 2010. Optimization of Media and Environmental Conditions for Alkaline Protease Production Using Bacillus subtilis in Submerged Fermentation Process. International Journal for Agro Veterinary and Medical Sciences 4 (4): 105-113.

Fuad, A.M., R. Rahmawati dan N.R. Mubarik. 2004. Produksi dan karakterisasi persial protease alkali termostabil Bacillus thermoglusidasius AF-01. Jurnal Mikrobiologi Indonesia 9 (1): 29-35.

Kumar, C. G. and H. Takagi. 1999. Microbial alkaline proteases from a bioindustrial viewpoint. Biotechnology Advance 17: 561-594.

Mabrouk, S.S., A.M. Hashem, N .M. A. El-Shayeb, A. M. S. Ismail and A. F. A. Fatah. 1999. Optimization of alkalin protease productivity by Bacillus licheniformis ATCC 21415. Bioresource Technology 69: 155-159.

Madigan, M. T., J. M. Martinko and J. Parker. 2000. Biology of Microorganisms. 9th Ed. Prenctice Hall International Inc. New Jersey.

Mubarik, N. R. 2001. Pemurnian dan Karakterisasi Protease ektraseluler dari isolat bakteri termofilik GP-04. Disertasi Institut Pertanian Bogor. Bogor.

Perez, A. M. 1999. Thermostability of powder enzymes: in vitro recoveries and in vivo efficacies. Proceedings of the Australian Poultry Science Symposium.

Rahman R. N. Z. R. A., M. Basri, and A. B. Salleh. 2003. Thermostable alkaline protease from Bacillus stearothermophilus F1; nutritional factors affecting protease production. Annals of Microbiology 53: 199-210.

Rakshit, S. K. and G. D. Haki. 2003. Development in industrially important thermostable enzymes: a review. Bioresource Technology. 89: 17–34.

Rao, C. S., S. S. Madhavendra, R. R. Sreenivas, P. J. Hobbs and R. S. Prakasham. 2008. Studies on improving the immobilized bead reusability and alkaline protease production by isolated immobilized Bacillus circulans (MTCC6811) using overall evaluation criteria. Applied Biochemistry and Biotechnology 150 (1): 65-83.

Rao, M. B., A. M. Tanksale, S. G. Mohini dan V. V.Deshpande. 1998. Molecular and Biotechnological Aspect of Microbial Proteases. Microbiology and Molecular Biology Reviews 62: 597- 635.

Razak, C. N. A., S. W. Tang., M. Basri., and A. B. Salleh. 1997. Preliminary Study on the Production of Extracellular Protease from a Newly Isolated Bacillus sp. (No.1) and the Physical Factors Mfecting Its Production. Pertanika Journal of Science and Technology 5 (2): 169-177.

Shafee, N., S. N. Aris, R. N. Z. A. Rahman, M. Basri and A. B. Saleh., 2005. Optimization of environmental and nutritional conditions for the production of alkaline protease by a newly isolated bacterium Bacillus cereus strains 146. Journal of Applied Sciences Research. 1(1): 1-8.

Singh, J., N. Batra, and R. C. Sobti. 2001. Serine alkaline protease from a newly isolated Bacillus sp. Process Biochemestry. 36: 781-785.

Soekopitojo, S. 1986. Pengaruh Inducer dan Kecepatan Agitasi tehadap poduksi Amiloglukosidase dari Aspergillus niger L 51/NRRL A-11, 264. Skripsi Sarjana Teknologi Pertanian. Institut Pertanian Bogor. Bogor.

Stanbury, P. F. and A. Whitaker. 1984. Principle of Fermentation Technology. Pergamon Press. . New York

Suhartono, M. T. 1989. Enzim dan Bioteknologi. PAU Bioteknologi IPB. Bogor.

Suhartono, M. T. 1991. Protease. PAU Bioteknologi IPB. Bogor.

Sumantha, A., C. Larroche, and A. Pandey. 2006. Microbiology and industrial of food-grade protease : A perspective. Food. Technology, Biotechnology, 44, 2, 211-220

Tarigan. 1998. Pengantar Mikrobiologi. Departemen Pendidikan. Yogyakarta.

Wahyuna, D. 2012. Isolasi dan Karakterisasi Bakteri Termo-proteolitik Sumber Air Panas Sungai Medang, Sungai Penuh, Jambi. Skripsi Sarjana Biologi Universitas Andalas (Unpublished).

Wardani A. K. dan L. O. Nindita. 2012. Purifikasi dan Karakterisasi Protease dari Bakteri Hasil Isolasi dari Whey Tahu. Jurnal Teknologi Pertanian 13 (3): 149- 156.

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How to Cite

Aznia, A., Agustien, A., & Nasir, N. (2015). Optimasi Parsial Isolat Termofilik M5-24 dalam Produksi Protease. Jurnal Biologi UNAND, 3(3). https://doi.org/10.25077/jbioua.3.3.%p.2014

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Vol. 3 No. 3 (2014)

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