Isolasi dan Karakterisasi Bakteri Alkali-Proteolitik Sumber Air Panas Semurup Kerinci Jambi
DOI:
https://doi.org/10.25077/jbioua.3.4.%25p.2014Abstract
Thermophile protease has economic value because this enzyme is useful on all kind of industry that used high temperature on it production process. In the effort of obtaining indigenous alkaline thermostable a protease bacterial that produce we explore potential hot spring at Semurup, Kerinci Jambi. This study was conducted from September 2013 to February 2014, Water samples were collected by purposive sampling, the character of thermophilic bacteria were described for each in laboratory of microbiology and biomolecular, development of biology, faculty of mathematic and natural science Andalas university, Padang. There were 40 thermophile colonies, 7 isolates showed alkali proteolytic index range from 0.3 to 2.00. SIII-10 isolate showed the highest in alkali proteolitic index. The characteristic of SIII-10 are: whute and bolt colony, gram negative, motil, positive sitrat agar and positive gelatin.
Key words, Isolation, characterization, alkaline protease, thermostable, Hot spring.
References
Brock, T.D. 1986. Introduction: An Overview of the Thermophiles,: general, Molecular and Applied Microbiology. Ed. T.D. Brock, A Wiley Interscience Publication, John Wiley and Sons, New York.
Cowan, D. 1992. Biochemistry and Molecular Biology of the Extremely Thermophilic Archaeabacteria, in : Molecular Biology and Biotechnology Extremophiles. Ed.R.A. Herbert and R.J.Sharp, Blackie and Sons. New York.
Edwards, C. 1990. Thermophiles in: Microbiology of Extreme Environments. Graw-Hill Publ. Company. New York.
Moon, S. H, dan S.J Parulekar. 1990. A parameter studi of protease production in batch and fed batch cultures of Bacillus. Biotech, Bioeng,, 37, 467-483
Naiola E dan Wihyastuti N. 2002. Isolasi, Seleksi dan Optimasi Produksi Protease dari beberapa Isolat Bakteri. Berita Biologi, 16 (3): 467-473.
Rajasa, H. 2003. Pidato Pembukaan 3nd conference on Industrial Enzyme and Biotechnology. Technology and bussines Opportunity for Industrial Enzyme in harmony with Enviroment. BPPT. Jakarta, 6-7 Oktober 2003.
Rilda, Y, dan Anthoni Agustien. 2004. Eksplorasi Bakteri-Bakteri Isolat Lokal Penghasil Enzim Serin-Alkali Protease. Jurnal Kimia Andalas, 10 (1) : 37-43-2004.
Sari. U. M. 2013. Penapisan dan Karakterisasi Bakteri Selulolitik Termofilik Sumber Air Panas Sungai Medan Kerinci Jambi. Skripsi Sarjana Biologi. Universitas Andalas. Padang.
Suwanto, A. 1994. Evolusi microbe dan kaitannya dengan sistematik molekuler. Hayati, 1, 2, 26-31.
Vermelho, A. B., M.N.L. Meirelles, A. Lopes, S.D.G. Petinate, A.A. Chaia, M.H. Branquinha. 1996. Detection of extracellular protease from microorganism on agar plates,https://www.bioline.org.br
Wahyuntari, B. 2001. Pemurnian dan Karakterisasi Protease Ekstraseluler Isolat Prokaroit Termofilik Ekstrim dari Tangkuban Perahu. Disertasi, IPB, Bogor
Ward, O. P. 1983. Proteinase. Di dalam Microbial Enzyme And Biotechnology. W. M.Fogarty. Applied Science Publisher. New York.
Downloads
Issue
Section
License
Please find the rights and licenses in Jurnal Biologi Universitas Andalas (JBioUA).
1. License
The non-commercial use of the article will be governed by the Creative Commons Attribution license as currently displayed on Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.Â
2. Author’s Warranties
The author warrants that the article is original, written by stated author(s), has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).
3. User Rights
JBioUA's spirit is to disseminate articles published are as free as possible. Under the Creative Commons license, JBioUA permits users to copy, distribute, display, and perform the work for non-commercial purposes only. Users will also need to attribute authors and JBioUA on distributing works in the journal.
4. Rights of Authors
Authors retain the following rights:
- Copyright, and other proprietary rights relating to the article, such as patent rights,
- The right to use the substance of the article in future own works, including lectures and books,
- The right to reproduce the article for own purposes, provided the copies are not offered for sale,
- The right to self-archive the article,
- the right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the article's published version (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal (Jurnal Biologi Universitas Andalas).
5. Co-Authorship
If the article was jointly prepared by other authors, they warrant that he/she has been authorized by all co-authors to sign this agreement on their behalf, and agrees to inform his/her co-authors of the terms of this agreement.
6. Royalties
This agreement entitles the author to no royalties or other fees. To such extent as legally permissible, the author waives his or her right to collect royalties relative to the article in respect of any use of the article by JBioUA or its sublicensee.
Privacy Statement
The names and email addresses entered in this journal site will be used exclusively for the stated purposes of this journal and will not be made available for any other purpose or to any other party.
However, due to the advancement and availability of hacking and data mining techniques found over the Internet, JBioUA will not be able to guarantee that other parties will not mine our users' email addresses in any possible ways found over the Internet.