Induksi Tunas Kunyit Putih (Curcuma zedoaria Roscoe) Pada Media MS Dengan Penambahan Berbagai Konsentrasi BAP dan Sukrosa Secara In Vitro
DOI:
https://doi.org/10.25077/jbioua.3.4.%25p.2014Abstract
Murashige-Skoog media enriched using 6-Benzyl Aminopurine (BAP) and sucrose was conducted from June 2013 to February 2014 at the Laboratory of Plant Physiology, Department of Biology, Andalas University. This study aimed to determine the ability of shoot formation of white turmeric (Curcuma zedaoria Roscoe) on the effect of BAP and sucrose concentration. We used a Completely Randomized Design (CRD) with 8 treatments and 3 replications, BAP (0, 1.5, 3 and 4.5 mg/L) and sucrose (3 and 5%) of MS medium. The result showed that the percentage of shoot induction was 100%. The best combination of sucrose and BAP in shoot induction was obtained on 5% sucrose and 1.5 mg/L BAP. While for the first immerge of shoot was obtained on 5% sucrose and 4.5 mg/L BAP in 5 days after inoculation. All treatments but did not give any significant effect to the number and height of shoots.
Key words: Curcuma zedoaria, shoot, sucrose and 6-Benzyl Aminopurine
References
Abidin, Z. 1985. Dasar-Dasar Pengetahuan Tentang Zat Pengatur Tumbuh. Angkasa. Bandung.
Anish, N. P., M. Dan dan M. Bejoy. 2008. Conservation Using In Vitro Progenies of the Threatened Ginger-Boesenbergia pulcherima (Wall.) Kuntze. International Journal of Botany. 4 (1): 93 – 98.
Bahera, K. K., D. Pani dan S. Sahoo. 2010. Effect of Plant Growth Regulator on In Vitro Multiplication of Tumeric (Curcuma longa L. cv. Rangga). International Journal of Biological Technology. 1 (1): 16 – 23.
Bhojwani, S.S. dan M.K. Rajdan. 1983. Plant Tissue Culture, Theory and Practise. Elsevier Scientific Pub Amsterdam.
Collin, H. A. dan S. Edward. 1998. Plant Cell Culture. BIOS Scientific Publisher. United Kingdom.
Dixon, R. A and R. A Gonzales. 1985. Plant Cell Culture A Practical Approach Second Edotion. Oxford University Press. New York.
------. 1995. Teknik Kultur In Vitro Dalam Hortikultura. PT. Penebar Swadaya. Jakarta.
George, E. F. and P.D. Sherington. 1984. Plant Propagation by Tissue Culture. Exegetics Ltd. England.
Hendaryono, D. P. S. dan A. Wijayani. 1994. Teknik Kultur Jaringan. Penerbit Kanisius. Yogyakarta.
Pierik, R. L. M. 1987. In Vitro Culture of Higher Plants. Martinus Nijhoff Publisher. Dodrecht.
Santoso, U. dan F. Nursandi. 2003. Kultur Jaringan Tanaman. UMM Pres. Malang.
Syukur, C. 2004. Temu Putih Tanaman Obat Anti Kanker. PT. Penebar Swadaya. Jakarta.
Syukur, C. dan Hernani. 2001. Budidaya Tanaman Obat Komersial. PT. Penebar Swadaya. Jakarta.
Yusnita. 2003. Kultur jaringan Cara Memperbanyak secara Efisien. Agromedia Pustaka. Jakarta.
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