Isolasi Bakteri Pendegradasi Hidrokarbon di Tanah Tercemar Lokasi Perbengkelan Otomotif

Authors

  • Hezi Yolantika Laboratorium Mikrobiologi, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Andalas, Kampus Limau Manis, Padang-25136
  • Periadnadi Periadnadi Laboratorium Mikrobiologi, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Andalas, Kampus Limau Manis, Padang-25136
  • Nurmiati Nurmiati Laboratorium Mikrobiologi, Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Andalas, Kampus Limau Manis, Padang-25136

DOI:

https://doi.org/10.25077/jbioua.4.3.%25p.2015

Keywords:

isolation, characterization, hydrocarbon degrading bacteria, lubricating oil, contaminated soil

Abstract

The research related to Isolation of the Hydrocarbon Degrading Bacteria in the Contaminated Soil of the Automotive Workshop was conducted from May to October 2014 at Laboratory of Microbiology, Biology Department, Faculty of Mathematics and Natural Sciences, Andalas University. The aimed of this study was to find  the bacteria able to degradate the hydrocarbon from the lubricating oil contaminated soil and to descripe characterization of isolates. This study used a survey method in isolation and characterization of hydrocarbon degrading bacteria.  The result obtained that the bacteria able to degradate the hydrocarbon from the lubricating oil contaminated soil about 39.02% (1.6x105 cfu/g). Five isolates were found (BTTO1, BTTO2, BTTO3, BTTO4 and BTTO5) has a rod shaped, Gram-negative, motile and positive catalase.

References

Arijanto dan B. Setyana. 2007. Pengujian Campuran Terbaik Bahan Bakar Alkohol-Bensin Ditinjau dari Aspek Kandungan Material Pelumas Pada Sepeda Motor 4 Langkah. Jurnal Rotasi 9(3): 40-42.

Cappucino, J. G dan N. Sherman. 2005. Microbiology a Laboratory Manual 7thEd. Pearson Education, Inc. Publishing as Benjamin Cummings. San Francisco. CA.

E Pertiwi, S., H. Widjajanti, B. Yudono, dan H. Wahyudi. 2011. Pemanfaatan Rumput Fimbrisylis sp. dalam Proses Bioremediasi Tanah pada Berbagai Konsentrasi Limbah Minyak Bumi. Jurnal Penelitian Sains 14(1): 57-61.

Hafiluddin. 2011. Bioremediasi Tanah Tercemar Minyak Dengan Teknik Bioaugmentasi dan Biostimulasi. Embryo 8(1): 47-52.

Hajar, D. 2012. Isolasi, Identifikasi dan Analisis Kemampuan Degradasi Hidrokarbon Bakteri Tanah Sampel B, Cilegon, Banten. [Skripsi]. Depok. Universitas Indonesia.

Hamdiyah, S. 2000. Isolasi dan Identifikasi Morfologi Bakteri Pendegradasi Minyak Bumi serta Efektifitasnya dalam Proses Bioremediasi. [Skripsi]. Bogor. Institut Pertanian Bogor.

Komarawidjaja, W. 2009. Karakteristik dan Pertumbuhan Konsorsium Mikroba Lokal Dalam Media Mengandung Minyak Bumi. Jurnal Teknik Lingkungan. 10(1): 114-119.

Mujab, A. S. 2011. Penggunaan Biokompos dalam Bioremediasi Lahan Tercemar Limbah Lumpur Minyak Bumi. [Skripsi]. Jakarta. Universitas Islam Negeri Syarif Hidayatullah.

Pitrandjalisari, V. 2009. Analisis Kelayakan Investasi Penggunaan Teknologi Crude Oil System di Departemen Power Plant PT Newmont Nusa Tenggara. Jurnal Teknik Industri 10(2): 109-113.

Sinaga, A. P. 2013. Perombakan Hidrokarbon dalam Tanah Terkontaminasi Minyak Berat, Minyak Ringan dan Oli Bekas oleh Bacillus sp. [Skripsi]. Bogor. Institut Pertanian Bogor.

Sugoro, I. 2002. Bioremediasi Sludge Limbah Minyak Bumi Lahan Tercemat dengan Teknik Land Farming dalam Skala Laboratorium. [Tesis]. Bandung. Institut Teknologi Bandung.

Surtikanti, H. dan W. Surakusumah. 2004. Studi Pendahuluan tentang Peranan Tanaman dalam Proses Bioremediasi Oli Bekas dalam Tanah Tercemar. Jurnal Ilmiah Biologi Ekologi dan Biodiversitas Tropika 2(1): 11-14.

Yojana, R. N. 1995. Aktivitas Bakteri Hasil Isolasi dari Tumpahan Minyak di Pelabuhan Dumai dalam Biodegradasi Hidrokarbon Minyak Bumi. [Skripsi]. Padang. Universitas Andalas.

Yoswaty, D. 2002. Pemanfaatan Bakteri Pemecah Minyak Dalam Proses Bioremediasi. [Tesis]. Jakarta. Universitas Indonesia.

Walker, J. D. dan Colwell. R. R. 1976. Enumeration of Petroleum-Degrading Microorganisms. Applied And Environmental Microbiology 31(2): 198-207.

Zam, S. I. 2011. Bioremediasi Tanah yang Tercemar Limbah Pengilangan Minyak Bumi Secara In Vitro pada Konsentrasi pH Berbeda. Jurnal Agroteknologi, 1(2): 1-8.

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Published

2015-09-15

How to Cite

Yolantika, H., Periadnadi, P., & Nurmiati, N. (2015). Isolasi Bakteri Pendegradasi Hidrokarbon di Tanah Tercemar Lokasi Perbengkelan Otomotif. Jurnal Biologi UNAND, 4(3). https://doi.org/10.25077/jbioua.4.3.%p.2015

Issue

Vol. 4 No. 3 (2015)

Section

Articles

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