Respon Pertumbuhan Nodus Artemisia vulgaris L pada Medium Murashige-Skoog dengan Penambahan Beberapa Zat Pengatur Tumbuh Secara In Vitro
DOI:
https://doi.org/10.25077/jbioua.4.3.%25p.2015Keywords:
Artemesia vulgaris L., plant growths regulator, in vitroAbstract
Artemesia vulgaris L produce artemisinin which is effective against resistant strains of Plasmodium falciparum, the malarial parasite. The study about growth responses of Artemesia vulgaris L in Murashige-Skoog (MS) medium supplemented with several growth regulators had been done from September to November 2014 at the Laboratory of Plant Phisiology and Tissue Culture, Department of Biology, Faculty of Mathematic and Natural Science, Andalas University. The aims of the study was to know the responses of node explants of Artemesia vulgaris L supplemented with several plant growth regulators for the effective technique of propagation. The study used Completely Randomized Design with four treatments and six replications. Node from in vitro growth were treated with three different kind of growth regulator (0.3 ppm BAP, 0.5 ppm NAA, 0.3 ppm GA3) in MS medium. The result showed that 0.5 ppm NAA was the best growth regulators formed callus, bud, and root of Artemesia vulgaris L.
Keywords: Artemesia vulgaris L, plant growths regulator, in vitro
References
Abidin, Z. 1994. Dasar-Dasar Pengetahuan Tentang Zat Pengatur Tumbuh. Penerbit Angkasa. Bandung.
Badan POM RI. 2008. Taksonomi Koleksi Tanaman Obat, Kebun Tanaman Obat Citeureup. Badan Pengawas Obat dan Makanan Republik Indonesia Deputi Bidang Pengawasan Obat Tradisional, Kosmetik, dan Produk Komplemen Direktorat Obat Asli Indonesia. Jakarta.
Ferreira, J. F. S. and J. Janick. 1996. Distribution of Artemisinin in Artemisia annua. In J. Janick (Ed.). Progress in New Crops. ASHS Press. Arlington.
Fitriani, H. 2008. Kajian Konsentrasi BAP Dan NAA Terhadap Multiplikasi Tanaman Artemisia Annua L. Secara In vitro. Skripsi Fakultas Pertanian Universitas Sebelas Maret. Surakarta.
George, E. F. and Sherrington, 1984. Plant Propagation by Tissue Culture Handbook and Directory of Commercial Laboratories Exegetics Ltd. Erasley. Bassingtone. England. 551pp.
Gomez, K. A. dan A. A . Gomez.1995. Prosedur Statistik untuk Pertanian. Edisi Kedua. Universitas Indonesia. Jakarta.
Gulati, A. S . Bharel, S. K. Jain, M. Z. Abdin. and Srivastawa P.S. 1996. In vitro micropropagation and flowering in Artemisia annua. J. Plant Biochem. Biotechnol. 5: 31-35.
Gunawan, L. W. 1987. Teknik kultur jaringan. Laboratorium Kultur jaringan Pusat Antar Universitas (PAU) Bioteknologi IPB: Bogor
Hendaryono, D. P. S dan A. Wijayani, 1994. Teknik kultur jaringan, pengenalan dan petunjuk perbanyakan tanaman secara vegetatif modern. Kanisius. Yogyakarta.
Lestari, E, G. 2011. Peranan Zat Pengatur Tumbuh dalam Perbanyakan Tanaman melalui Kultur Jaringan. Jurnal AgroBiogen 7(1):63-68.
Liu C. Z., S. J. Murch, and M. El-Demerdash. (2003) Regeneration of Egyptian medicinal plant Artemisia judaica L. Plant Cell Rep. 21(6): 525-530.
Marlina, N dan D. Rusnandi. 2007. Teknik Aklimatisasi Planlet Anthurlum Pada Beberapa Media Tanam. Buletin Teknik Pertanian Vol. 12 No. 1.
Noggle, G. R and G. J. Fritz. 1979. Introductory Plant Physiologi. Second Edition. Perentice Hall of India Private LTD. New Delhi.
Nurhayati, H. dan Gusmaini. 2007. Potensi Pengembangan Budidaya Artemisia annua L. di Indonesia. Perspektif Vol. 6 No. 2 / Desember 2007. Hal 57 – 67 ISSN: 1412-8004.
Nursetiadi, E. 2008. Kajian Macam Media dan Konsentrasi BAP Terhadap Multiplikasi Tanaman Manggis (Garcinia Mangostana L.) Secara In Vitro. Skripsi Jurusan Agronomi Fakultas Pertanian Universitas Sebelas Maret Surakarta.
Prawiranata, W., H. Said, dan T. Pin. 1995. Dasar-dasar Fisiologi Tumbuhan. Jilid 2. Departemen Botani Fakultas Matematika dan IPA IPB: Bogor.
Santoso, U. dan F. Nursandi, 2004. Kultur Jaringan Tanaman. Universitas Muhammadiyah Malang Press. Malang.
Setiawati, W., R . Murtaningsih, N., Gunaeni, dan T. Rubiati. 2008. Tumbuhan Bahan Pestisida Nabati dan Cara Pembuatannya Untuk Pengendalian Organisme Pengganggu Tumbuhan (OPT). Prima Tani Balitsa ISBN: 978-979-8304-53-8. Balai Penelitian Tanaman Sayuran Pusat Penelitian dan Pengembangan Hortikultura Badan penelitian dan Pengembangan Pertanian. Bandung.
Wattimena, G. A. 1987. Zat Pengatur Tumbuh Tanaman. Pusat Antar Universitas Bioteknologi. Institut Pertanian Bogor. Bogor. 247 pp.
Yunita, R,. E. G. Lestari. 2008. Komunikasi Pendek. Perbanyakan Tanaman Artemisia Annua Secara In vitro. Jurnal Agrobiogen 4(1):41-44.
Yusnita. 1997. Pengaruh BAP, Medium Dasar dan Jenis Eksplan terhadap Pembentukan Tunas Melinjo (Gnetum gnemon L.) Secara In vitro. Jurnal grotopika. 2(2) : 29-3
Downloads
How to Cite
Issue
Section
License
Please find the rights and licenses in Jurnal Biologi Universitas Andalas (JBioUA).
1. License
The non-commercial use of the article will be governed by the Creative Commons Attribution license as currently displayed on Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
2. Author's Warranties
The author warrants that the article is original, written by stated author(s), has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).
3. User Rights
JBioUA's spirit is to disseminate articles published are as free as possible. Under the Creative Commons license, JBioUA permits users to copy, distribute, display, and perform the work for non-commercial purposes only. Users will also need to attribute authors and JBioUA on distributing works in the journal.
4. Rights of Authors
Authors retain the following rights:
• Copyright, and other proprietary rights relating to the article, such as patent rights,
• The right to use the substance of the article in future own works, including lectures and books,
• The right to reproduce the article for own purposes, provided the copies are not offered for sale,
• The right to self-archive the article,
• the right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the article's published version (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal (Jurnal Biologi Universitas Andalas).
5. Co-Authorship
If the article was jointly prepared by other authors, they warrant that he/she has been authorized by all co-authors to sign this agreement on their behalf, and agrees to inform his/her co-authors of the terms of this agreement.
6. Royalties
This agreement entitles the author to no royalties or other fees. To such extent as legally permissible, the author waives his or her right to collect royalties relative to the article in respect of any use of the article by JBioUA or its sublicensee.
Privacy Statement
The names and email addresses entered in this journal site will be used exclusively for the stated purposes of this journal and will not be made available for any other purpose or to any other party.
However, due to the advancement and availability of hacking and data mining techniques found over the Internet, JBioUA will not be able to guarantee that other parties will not mine our users' email addresses in any possible ways found over the Internet.