Bioaktivitas Antibakteri Lamun Thalassia hemprichii dan Enhalus acoroides

Authors

  • Arief Anthonius Purnama Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Pasir Pengaraian, Kabupaten Rokan Hulu 28557, Riau
  • Eti Meirina Brahmana Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Pasir Pengaraian, Kabupaten Rokan Hulu 28557, Riau

DOI:

https://doi.org/10.25077/jbioua.6.1.45-50.2018

Keywords:

Antibacterial, Bioactivity, Enhalus acoroides, Thallasia hempricii.

Abstract

The objective of present study was to identify bioactivity of antibacterial Indonesian Seagrass Thalassia hemprichii dan Enhalus acoroides from Karang Tirta Beach, Padang City, West Sumatera. This is a preliminary research for a new alternative antibiotic from Seagrass in Indonesia. In this study the seagrass was examined by a maceration process and using the rotary evaporator to obtain viscous extract. The material for the bioactivity testing was performed upon several intended bacteria such as Escherichia coli, Stapillococcus aureus, and Bacillus subtilis. The bioactivity testing was performed by using the method of resazurin and MIC (Minimum Inhibitory Concentration), which is determined by looking at well blue-colored at the smallest concentration. The result revealed that the best antibacterial bioactivity of Thalassia hemprichii extract was a total of 62.50 μg/mL N-hexane solvent against Escherichia coli and Stapillococcus aureus bacteria. While the best antibacterial bioactivity of Enhalus acoroides extract is 15.62 μg/mL of N-hexane solvent against Stapillococcus aureus.

References

Azwanida, N, N. 2015. A Review On The Extraction Methods Use In Medical Plants, Principle, Strength and Limitation. Med Aromatic Plants 4: 196-201.

Bwanga, F., Moses, J., Melles, H., Sven, H. 2010. Evaluation of seven tests for the rapid detection of multidrug- resistant tuberculosis in Uganda. Int. J. Tuberc. Lung Dis 14: 890–895.

Bjork, M., Fred, S., Elizabeth, M., Sven, B. 2008. Managing Seagrasses for Resilience to Climate Change. IUCN. Gland. Switzerland.

El Din., Nihal G., Shams., Zeinab M., El- Sherif. 2013 "Nutritional value of Cymodocea nodosa and Posidonia oceanica along the western Egyptian Mediterranean coast. " The Egyptian Journal of Aquatic Research 393: 153-165.

Green, P. E dan F. T, Short. 2003. World Atlas of Seagrasses. Prepared by the UIMEP World Conservation Monitoring Centre. University of California Press, Berkeley. USA.

Jawetz., Melnick., Adelberg’s., 2005 Medical Microbiology. Salemba Medika, Jakarta.

Kannan, R, R., Arumugam, R., Anantharaman, P. 2010. Antibacterial Potential Of Three Seagrasses Against Human Pathogens. Asian Pasific Journal Of Tropical Medicine: 890-893.

Kannan, R, R., Arumugam, R, Iyapparaj, P., Thangaradjou, T., Anantharaman, P. 2013. In Vitro Antibacterial, Cytotoxity and Haemolytic Activities and Phytochemical Analysis Of Seagrasses From The Gulf Of Mannar, South India. Food Chemistry 136: 1484-1489.

Kuo, J. 2007. New Monoecious Seagrass of Hallophila sulawesii (Hidrocharitaceae) from Indonesia. Aqauat Bot 87: 171-175.

Kuriandewa, T. E., Supriyadi I. H. 2006. Seagrass Mapping in East Bintan Coastal Area, Riau Archipelago, Indonesia, Indonesia. Coastal Marine Science 30 (1): 154-161.

Mani, A. E. Velammal, A dan Jamila, P. 2012. Phytochemicals of The Seagrass Syringodium Isoetifolium and Its Antibacterial And Insecticidal Activities, European Journal of Biological Sciences 4 (3): 63-67.

Martin, A., Camacho, M., Portaels, F., Palomino, J. C. 2003. Resazurin

microtiter assay plate testing of Mycobacterium tuberculosis susceptibilities to second-line drugs: rapid, simple, and inexpensive method. Antimicrob Agents Chemother 47 (11): 3616-3619.

Pandey, A., Shalini, T. 2014. Concept Of Standardization, Extraction and Pre Phytochemical Screening Strategies For Herbal Drug. Journal Of Pharmocognosy and Phytochemical 2 (5): 115-119.

Petrussa, E., Braidot, E., Zancani, M., Peresson, C., Bertolini, A., Patui, S., Vianello, A. 2013. Plant Flavonoids Biosynthessis, Transport and Involvement in Stress Responses. International Journal of Molecular Sciences 14 14950-14973.

Purnama, A. A., Zakaria, I. J., Nurdin, J. 2014. The Diversity And Distribution Of Seagrass In Karang Tirta Beach Padang City, West Sumatera. Prosiding International Conference Shiite 4th Annual Syiahkuala University (AIC -UNSYIAH) in conjunction With The 9th Annual International Workshop and Expo on Sumatra Tsunami Disaster and Recovery,

Universitas Syiah Kuala, Banda Aceh, 22 – 24 Desember 2014. Syiah Kuala University Press. pp: 172-176.

Rampersad, S.N. 2012. Multiple Applications of Alamar Blue as an Indicator of Metabolic Function and Celluar Health in Cell Viability Bioassays. J. Sensors: 12347-12360.

Rompas, R. A., Hosea, J. E., Adithya, Y. 2012. Isolation and Identification Of Flavonoids In Seagrass Leaf (Syringodium isoetifolium). PHARMACON 1(2): 222 - 227

Ravikumar, S., Thajuddin, N., Suganthi, P., Jacob Inbaneson, S., and Vinodkumar, T. 2010. Bioactive potential of seagrass bacteria against human bacterial pathogens. Journal of Environmental Biology 31(3): 387 - 392.

Ravikumar, S., Ali, M. S., Anandh, P., Ajmalkhan, M., and Dhinakaraj, M. 2011. Antibacterial activity of Cymodocea serrulata root extract against chosen poultry pathogens. Indian Journal of Science and Technology 4(2): 98-100.

Sani, R. N., Nisa, F. C., Andriani. R.D and Maligan, J. M. 2014. Yield Analysis and Phytochemical Screening Ethanol Extract of Marine Microalgae Tetraselmis Chui. Jurnal Pangan dan Agroindustri 2(2): 121-126.

Sarker, S. D., Lutfun, N., Yashodharan, K. 2007. Microtitre Plate-Based Antibacterial Assay Incorporating Resazurin as an Indicator of Cell Growth, and Its Application in the in Vitro Antibacterial Screening of Phytochemicals. Methods 42: 321 – 24.

Subhashini, P., Elangovan, D., Thirunavukkarasu, T., Jutta, P. 2013, Bioactive Natural Products From Marine Angiosperm: Abundance and Functions, Natural Product Bioprospect: 129 - 136.

Steele, L., Melanie, C., Anne, B., Tom, A. 2015. "Seagrass–pathogen interactions: ’pseudo induction’of turtlegrass phenolics near wasting disease lesions." Marine Ecology Progress Series 303: 123-131.

Umamaheswari, M., Asokkumar, K., Sivashanmugam , A. T., Remyaraju, A, Subhadradevi, V., Ravi, T.K. In vitro xanthine oxidase inhibitory activity of the fractions of Erythrina stricta Roxb. Journal of ethnopharmacology 124(3): 646-648.

Downloads

Published

2018-03-07

How to Cite

Purnama, A. A., & Brahmana, E. M. (2018). Bioaktivitas Antibakteri Lamun Thalassia hemprichii dan Enhalus acoroides. Jurnal Biologi UNAND, 6(1), 45–50. https://doi.org/10.25077/jbioua.6.1.45-50.2018

Issue

Vol. 6 No. 1 (2018)

Section

Articles

License

Please find the rights and licenses in Jurnal Biologi Universitas Andalas (JBioUA).

1. License
The non-commercial use of the article will be governed by the Creative Commons Attribution license as currently displayed on Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

2. Author's Warranties
The author warrants that the article is original, written by stated author(s), has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).

3. User Rights
JBioUA's spirit is to disseminate articles published are as free as possible. Under the Creative Commons license, JBioUA permits users to copy, distribute, display, and perform the work for non-commercial purposes only. Users will also need to attribute authors and JBioUA on distributing works in the journal.

4. Rights of Authors
Authors retain the following rights:
• Copyright, and other proprietary rights relating to the article, such as patent rights,
• The right to use the substance of the article in future own works, including lectures and books,
• The right to reproduce the article for own purposes, provided the copies are not offered for sale,
• The right to self-archive the article,
• the right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the article's published version (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal (Jurnal Biologi Universitas Andalas).

5. Co-Authorship
If the article was jointly prepared by other authors, they warrant that he/she has been authorized by all co-authors to sign this agreement on their behalf, and agrees to inform his/her co-authors of the terms of this agreement.


6. Royalties
This agreement entitles the author to no royalties or other fees. To such extent as legally permissible, the author waives his or her right to collect royalties relative to the article in respect of any use of the article by JBioUA or its sublicensee.


Privacy Statement
The names and email addresses entered in this journal site will be used exclusively for the stated purposes of this journal and will not be made available for any other purpose or to any other party.
However, due to the advancement and availability of hacking and data mining techniques found over the Internet, JBioUA will not be able to guarantee that other parties will not mine our users' email addresses in any possible ways found over the Internet.