Induksi Embriogenesis Somatik Pada Anggrek Vanda Sumatrana Schltr. dengan Penambahan Beberapa Konsentrasi Asam 2,4-Diklorofenoksiasetat (2,4-D)
DOI:
https://doi.org/10.25077/jbioua.7.1.6-13.2019Keywords:
2, 4-D, Somatic Embryogenesis, Vanda sumatrana Schltr.Abstract
The research about induction of somatic embryogenesis of Orchid Vanda sumatrana Schltr. by giving 2,4-Diclorophenoxiacetic acid (2,4-D), was conducted from May to July 2016 in plant Physiology and tissue Culture Laboratory, Biology Departement, Matematics and Natural Science Faculty, Andalas University. The aim of this study was found the concentration of 2,4-D to induce somatic embryogenesis of Vanda sumatrana. The research used Completely Randomized Resign (CDR) with 6 treatments and 4 replication. The treatments were : without 2,4-D (control); 1 mg/L; 2 mg/L; 3 mg/L; 4 mg/L; 5 mg/L. The result showed that 2 mg/L 2,4-D and 3 mg/L 2,4-D were concentrations to induct somatic embryogenesis.
References
Arditti, J. and Ernst, R. 1993. Micropropagation of Orchid. John Wiley& Sons Inc. New York.
Collin, H.A dan Edwad.S. 1998. Plant cell culture. BIOS Scientific Publisher. United Kingdom.
Comber, J. B. 2001. Orchids of Sumatra. The Royal Botanic Garden. Kew.
Deli, R.N, Noli, Z.A dan Suwirmen. 2015.respon Pertumbuhan Nodus Artemesia vulgaris L. Pada medium Murashige-Skoog dengan Penambahan Beberapa Zat Pengatur Tumbuh Secara In vitro. Jurnal Biologi Universitas Andala. 4(3) :162-168.
Dwiyani,. R. 2013. Induksi Kalus pada Tanaman Anggrek Vanda tricolor Lindl. Var. Suavis Upaya Penyediaan Target Transformasi Melalui Argobacterium tumefaciens. Jurnal Agrotropika. 18(2) : 73-76.
Edy, A dan Pujisiswanto, H. 2008. Pengaruh 2,4-D terhadap Induksi Embrio Somatik Eksplant Laevet pada Beberapa Varietas Kacang Tanah (Arachis hipogea) Secara in vitro. Prosiding Seminar Nasional Sains dan Teknologi II Universitas Lampung. : 17-18
Fadhilah, N. 2016. Induksi Embriogenesis somatik Artemesia vulgaris L. Dengan pemberian 2,4-D.{Skripsi}. Universitas Andalas. Padang.
Fintarti, M. 2010. Embriogenesis Somatik dari Kalus Peganggan (Centella asiantica L Urban) Dengan Pemberian 2,4-D. {Skripsi}. Universitas Andalas. Padang.
Gardner, F, P. Pearce,R. B, dan R. I, Mitchell. 1991. The Plantation of Vegetation Physicology. Academic press. London
George E.F., Hall M.A., Jan De Clerk G. 2008. Plant Propagation by Tissue Culture 3rd edition. Volume 1. The background. Springer. P: 183-197.
Hendaryono, D.P.S., dan A.Wijayani. 1994. Teknik Kultur Jaringan. Penerbit Kanisius. Yogyakarta. 139p.
Hoesen, D.S.H., Widjaksono., dan Sukamto LA. 2008. Induksi kalus dan Organogenesis Kultur/ZV-Vitro Dendrobium lineale Rofle. Berita Biologi 9(3) : 333-341.
Indah., P. N dan Ermavitalini, D. 2013. Induksi Kalus Daun Nyamplung (Calophyllum inophylum Linn.) pada Beberapa Kombinasi Konsentrasi 6-Benzylaminopurine (BAP) dan 2,4-Dichlorophenoxyacetic acid (2,4-D). Juranal Sains dan Seni POMITS. 2 (1): 2337-3520
Indarjo, S. 2003. Pengaruh pemberian zat ppengatur tumbuh NAA dan 2,4-D terhadap pembentukan kalus pada kultur invitro polen anggrek Dendrobium jakarta molek. {Skripsi}. Fakultas kehutanan. IPB. Bogor.
Indrianto, A. 2003. Kultur Jaringan Tumbuhan. Fakultas Biologi Universitas Gadjahmada, Yogyakarta.
Klaus dan Haensch.T. 2007. Influence of 2,4-D and BAP on callus growth and the subsequent regeneration of somatic embryos in long-term cultures of Pelargonium X Domesticum Cv. Madame Layal. Electronic Journal of Biotechnology 10(1): 69 – 77.
Latif, S.M. 1960. Bunga anggerik permata belantara indonesia. Sumur bandung. Bandung
Lestari, E.G. 2011. Peranan Zat Pengatur Tumbuh dalam PerbanyakanTanaman melalui Kultur Jaringan. Jurnal AgroBiogen 7(1):63-68
Lizawati, Neliyati, R., Desrfira. 2012. Induksi Kalus Eksplan Daun Durian (Durio Zibethinus Murr cv Selat Jambi) pada beberapa kombinasi 2,4-D
dan BAP. 2012. Jurnal Online UNJA. 1 (1) : 2302-6472
Mariska, I., D. Sopandie, S. Hutami, E. Syamsudin, dan M. Kosmiatin. 2001. Peningkatan ketahanan terhadap Al pada tanaman kedelai melalui kultur in vitro. Laporan Riset Unggulan Terpadu VIII. Kantor Menristek dan LIPI, Jakarta.
Naing, A.H., J.D Chung dan K.B. Lim. 2011. Plant Regeneration throuhgt Indirect Somatic Embryogenesis in Coelogyne cristata Orchids. American Journal Of Plant Science. 2: 262-267.
Narayanaswamy. 1994. Plant Cell and tissue Culture . Tata Mc Graw-Hill Publishing Company Limited. New Delhi.
Oktavia,. Fetrina,. Siswanto,. Budiani dan sudarsono. 2003. Embriogenesis somatik langsung dan regenerasi planlet kopi Arabika (coffea arabika) dari Berbagai Eksplan. Menara perkebunan 2003. 71 (02) : 45-55.
Pierik, R.L.M. 1987. In Vitro Of Higher Plants.Martunis Nijhoff. Publ.Dordrecht.344
Purmaningsih, R. 2004. Regenerasi tanaman melalui embriogenesis somatik dan beberapa gen yang mengendalikannya. Buletin Agrobio
Balai penelitian Bioteknologi Tanaman Pangan. Bogor. 5(2):51-58.
Rahayu, B., Solichatun, dan E. Anggarwulan. 2003. Pengaruh Asam 2,4-Diklorofenoksiasetat (2,4-D) terhadap Pembentukan dan Pertumbuhan Kalus serta Kandungan Flavonoid Kultur Kalus Acalypha indica L. Biofarmasi .1(1) :1-6.
Rianawati, S. Purwito, A. Marwoto, B. Kurniati, R dan Suryanah. 2009. Embriogenesis somatik dari eksplan daun Phalaenopsis sp L. J. Argon. Indonesia. 37(3):240-248.
Santoso, U. Dan Nursandi. F. 2004. Kultur jaringan tanaman. UMM Pers. Malang.
Sriyanti, D.P. 2000. Pelestarian Tanaman Nilam (Pogostemon heyneasus Benth.) melalui Kultur Mikrostek. Biosmart 2(2): 19-22.
Suwirmen. 2009. Induksi dan Multiplikasi Tunas Tumbuhan Andalas (Morus macroura Miq., var. macroura) secara In vitro dalam Konservasi Plasma Nutfah Mascot Flora Sumatra Barat. Berk. Penel. Hayati Edisi Khusus: 3D : 61–65
Utami, E.S.W., Sumardi, I., Taryono, dan . Semiarti, E. 2007. Pengaruh α- Naphtaleneacetic Acid (NAA) Terhadap Embriogenesis Somatik Anggrek Bulan Phalaenopsis Amabilis (L.) Bl. Jurnal Biodiversitas. 8 (4) : 295-299.
Wattimena, G. A, L. W Gunawan, N. Mattjik, A, Syamsudin, E, Wiendi, N. M.
A Ermwati. 1991. Bioteknologi Tanaman. PAU Bioteknologi. IPB Bogor.
Widuri LI, Dewanti, P. Soeparjono, S. 2015. Induksi somatik embriogenesis tanaman tebu transgenik SUT event 02 menggunakan 2,4-D dan BAP. Berkala Ilmiah Pertanian 1(1) : 1-5.
Yelnititis. 2012. Pembentukan Kalus Remah dari Eksplan Daun Ramin (Gonystylus bancanus (Miq) Kurz). Jurnal Pemuliaan Tanaman Hutan 6:181-194.
Zaid, A. 1987. Invitro Browning of Tissue and Media With Special Emphasis To Date Palm Culture. Acta Holticulture 21( 2): 561-566.
Downloads
Published
How to Cite
Issue
Section
License
Please find the rights and licenses in Jurnal Biologi Universitas Andalas (JBioUA).
1. License
The non-commercial use of the article will be governed by the Creative Commons Attribution license as currently displayed on Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
2. Author's Warranties
The author warrants that the article is original, written by stated author(s), has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).
3. User Rights
JBioUA's spirit is to disseminate articles published are as free as possible. Under the Creative Commons license, JBioUA permits users to copy, distribute, display, and perform the work for non-commercial purposes only. Users will also need to attribute authors and JBioUA on distributing works in the journal.
4. Rights of Authors
Authors retain the following rights:
• Copyright, and other proprietary rights relating to the article, such as patent rights,
• The right to use the substance of the article in future own works, including lectures and books,
• The right to reproduce the article for own purposes, provided the copies are not offered for sale,
• The right to self-archive the article,
• the right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the article's published version (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal (Jurnal Biologi Universitas Andalas).
5. Co-Authorship
If the article was jointly prepared by other authors, they warrant that he/she has been authorized by all co-authors to sign this agreement on their behalf, and agrees to inform his/her co-authors of the terms of this agreement.
6. Royalties
This agreement entitles the author to no royalties or other fees. To such extent as legally permissible, the author waives his or her right to collect royalties relative to the article in respect of any use of the article by JBioUA or its sublicensee.
Privacy Statement
The names and email addresses entered in this journal site will be used exclusively for the stated purposes of this journal and will not be made available for any other purpose or to any other party.
However, due to the advancement and availability of hacking and data mining techniques found over the Internet, JBioUA will not be able to guarantee that other parties will not mine our users' email addresses in any possible ways found over the Internet.
