Callus Induction of Aerides odorata Lour. by Adding 2,4 Dichlorophenoxyacetic Acid (2,4-D)
DOI:
https://doi.org/10.25077/jbioua.7.2.109-117.2019Keywords:
2, 4-D, Aerides odorata, callusAbstract
The research about callus induction of Aerides odorata L. by adding 2,4 Dichlorophenoxyacetic Acid (2,4-D), has been done from August to October 2018 in Plant Physiology and Tissue Culture Laboratory, Department of Biology, Faculty of Mathematics and Natural Science, Andalas University, Padang. The aim of this research was found the effective consentration of 2,4-D to induce somatic embryo of A.odorata. The research used Completely Randomized Design (CRD) with 5 treatments and 6 replications. The treatments were: 0 mg/L 2,4-D; 0,25 mg/L 2,4-D; 0,5 mg/L 2,4-D; 0,75 mg/L 2,4-D; 1 mg/L 2,4-D. The result showed that the treatmeant were able induction callus of A.odorata, with compact until the friable texture, color of the resulting callus is yellowish green and greenish yellow. 2,4-D 1 mg/L was the best concentration to increase fresh weight of callus.
References
Astuti, A.T. 2016. Induksi Embriogenesis Somatik Pada Vanda sumatrana Schltr. dengan Berbagai Konsentrasi Asam 2,4-Diklorofenoksiasetat (2,4-D). [Skripsi]. Padang. Universitas Andalas.
Azmi, T.K.W dan N.M.A. Wiendi. 2013. Perbanyakan anggrek spesies Paphiopedilum glaucophyllum J.J.Smith melalui Proliferasi Tunas Adventif Secara In vitro. J.Hort Indonesia 4(3): 115-123.
Balilashaki, K., M.Vahedi., dan R. Karimi. 2015. In vitro direct regeneration from node and leaf explant of Phalaenopsis cv. Surabaya. Plant Tissue Culture & Bitechnology 25(2): 193ï€205.
Chaireok, S. 2015. Cryopreservation of Protocorm Like Bodies and Callus of Lady’s slipper orchids (Paphipedilum niveum (Rchb.f.) Stein) by vitri-vication and encapsulation vitri-vication.[Thesis].Songkla University.
Devi, H.S., S.I. Devi dan T.D. Singh. 2013. High Frequency Plant Regeneration Sydtem of Aerides odorata Lour. Through Foliar And Shoot Tip Culture. Horti Agrobo 41(1):169-176.
Dzuraibak,R.F. 2014. Inisiasi Dan Proliferasi Kalus Serta Induksi
Kalus Embriogenik Pada Kultur Antera Kedelai (Glycine max L. Merrill). [Thesis]. Program Studi Biologi Tumbuhan. Institut Pertanian Bogor. Bogor.
Fadhilah, N., Z.A. Noli dan Suwirmen. 2015. Induksi Kalus Artemisia vulgaris L. dengan Pemberian Beberapa Konsentrasi 2,4 Dichloro-phenoxyacetic Acid (2,4-D). Jurnal Biologi Universitas Andalas 4(4): 216-222.
Fintarti, M. 2010. Embriogenesis Somatik dari Kalus Pegagan (Centella asiatica L. Urban) dengan Pemberian 2,4-Diklorofenoksiaasetat (2,4-D). [Skripsi]. Padang. Universitas Andalas.
George, E. F. dan Sherrington. 1984. Plant Propagation by Tissue Culture.
Eastern Press. Reading Berks.
George, E.F., M.A. Hall dan G.J. De Klerk. 2008. Plant Propagation by Tissue Culture 3rd Edition vol 1. Springer. Netherlands.
Hardjo, P.H. 2016. Proliferasi PLBs Vanda tricolor Lindl. var. Pallida. Prosiding Seminar Nasional Biologi. ISBN: 978-602-951-11-9.
Indah, P.N dan D. Ermavitalini. 2013. Induksi Kalus Daun nyamplung (Calophyllum inophylum Linn.) pada Beberapa Kombinasi Konsentrasi 6-Benzylaminopurine (BAP) dan 2,4-Dichlorophenoxyacetic acid (2,4-D). Jurnal Sains dan Seni Pomits 2 (1).
Kaewubon, P., S.Sangdam, Thammasiri, Kanchit dan U.Meesawat. 2010. Plant Regeneration through Somatic Embryogenesis from Callus-derived PLBs of Tropical Slipper Orchid (Paphiopedilum niveum) (Rchb.f.) Pfitz.). Floriculture and Ornamental Biotechnology 4( 1) 29-35.
Lerch, K. 1981. Tyrosinase Kinetics A Semi Quantitative Model of Mechanism Of Oxidation of Monohydric And Dihydric Phenolic Substrates. Dalam Hutami, S. Ulasan Masalah Pencolatan Pada Kultur Jaringan. AgroBiogen 4(2):83-88.
Mahadi, I., S. Wulandari dan A. Omar. 2014. Pengaruh Naftalen Acetyl Acid (NAA) dan Benzyl Amino Purin (BAP) Terhadap Pembentukan Kalus Tanaman Rosella (Hibiscus sabdariffa). Jurnal Biogenesis 11(1): 1-7.
Mahadi, I., W.Syafi’i dan Y.Sari .2016. Induksi Kalus Jeruk Kasturi (Citrus microcarpa) Menggunakan Hormon 2,4-D dan BAP dengan Metode in vitro. Jurnal Ilmu Pertanian Indonesia (JIPI) 21 (2): 84ï€89.
Naing, A.H., J.D.Chung dan Ki B. Lim. 2011. Plant Regeneration Through Indirec Somatic Embryogenesis in Coelogyne cristata Orchid. American Journal of Plant Sciences 2. 262-267.
Netty, WS. 2010. Kultur Jaringan Tumbuhan. Universitas Andalas. Padang.
Purnamaningsih, R. 2002. Regenerasi Tanaman Melalui Embrio Somatik dan Beberapa Gen yang Mengenda-likannya. Balai Penelitian Biotek-nologi dan Sumber Daya Genetik Pertanian. Buletin Angrobio 5(2):51-58.
Samudin, S. 2009. Pengaruh Kombinasi Auksin-Sitokinin Terhadap Pertum-buhan Buah Naga. Media Litbang Sulawesi Tengah. Vol II. No. 1. Badan Penelitian dan Pengem-bangan Daerah Provinsi Sulawesi Tengah.
Smith,R.H. 1992. Plant Tissue Culture: Techniques and Experiments. Acadenic Press Inc. New York.
Sulistiarini,D. 2008. Keanekaragaman Jenis Anggrek Pulau Wawonii. Berk. Penel. Hayati. 14:21–27.
Robles-Martinez, M., A.P. Barba-de la Rosa, F.Gueroud, A.Negre Salvayre,M.Rossognol,M.S.Santos-Diaz. 2016. Establishment of Callus and Cell Suspensions of Wild and Domesticated Opuntia Species: Study on Their Potential as A Source of Metabolite Production. Plant Cell Tissue and Organ Culture 124(1): 181ï€189.
Rachmawati., Purwito., Wiendi., Mattjik dan Winarto. 2014. Perbanyakan Massa Anggrek Dendrobium Gradita 10 Secara In Vitro Melalui Embriogenesis Somatik. J. Hort 24(3):196-209.
Trisnawarti, N dan N.I. Sumardi. 2000. Kultur Ovule Jeruk Nipis (Citrus aurantifolia)Terhadap Keberhasilan Embriogenesis Somatik. Balai Penelitian Buah. Solok.
Utami, E.S.W., I. Sumardi, Taryono, dan E. Semiarti. 2007. Embriogenesis Somatik Anggrek Bulan (Phalaeonopsis amabilis L.) Struktur dan Pola Perkembangan. Berk. Penel. Hayati 13(33-38).
Wahyuningtiyas, L., R. S. Resmisari, Ach. Nashichuddin. 2014. Induksi Kalus Akasia (Acacia mangium) dengan Penambahan Kombinasi 2,4-D dan BAP Pada Media MS. Jurnal Biologi Universitas Islam Negeri Maulana Malik Ibrahim.
Wulansari, A., A.F. Martin., D.E. Rantau dan T.M. Ermayanti .2013. Perbanyakan Beberapa Aksesi Talas (Colocasia esculenta L.) Diploid Secara Kultur Jaringan dan Konservasinya Mendukung Diver-sivikasi Pangan. Seminar Nasional Riset Pangan, Obat-Obatan Dan Lingkungan Kesehatan. LIPI.
Yelnititis. 2012. Pembentukan Kalus Remah dari eksplan daun ramin (gonystylus bancanus (Miq) Kurz.). Jurnal pemuliaan tanaman hutan.
Zulkarnain, H. 2009. Kultur Jaringan Tanamans:Solusi Perbanyakan Tanaman Budidaya. PT Bumi Aksara. Jakarta.
Downloads
Published
How to Cite
Issue
Section
License
Please find the rights and licenses in Jurnal Biologi Universitas Andalas (JBioUA).
1. License
The non-commercial use of the article will be governed by the Creative Commons Attribution license as currently displayed on Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
2. Author's Warranties
The author warrants that the article is original, written by stated author(s), has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).
3. User Rights
JBioUA's spirit is to disseminate articles published are as free as possible. Under the Creative Commons license, JBioUA permits users to copy, distribute, display, and perform the work for non-commercial purposes only. Users will also need to attribute authors and JBioUA on distributing works in the journal.
4. Rights of Authors
Authors retain the following rights:
• Copyright, and other proprietary rights relating to the article, such as patent rights,
• The right to use the substance of the article in future own works, including lectures and books,
• The right to reproduce the article for own purposes, provided the copies are not offered for sale,
• The right to self-archive the article,
• the right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the article's published version (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal (Jurnal Biologi Universitas Andalas).
5. Co-Authorship
If the article was jointly prepared by other authors, they warrant that he/she has been authorized by all co-authors to sign this agreement on their behalf, and agrees to inform his/her co-authors of the terms of this agreement.
6. Royalties
This agreement entitles the author to no royalties or other fees. To such extent as legally permissible, the author waives his or her right to collect royalties relative to the article in respect of any use of the article by JBioUA or its sublicensee.
Privacy Statement
The names and email addresses entered in this journal site will be used exclusively for the stated purposes of this journal and will not be made available for any other purpose or to any other party.
However, due to the advancement and availability of hacking and data mining techniques found over the Internet, JBioUA will not be able to guarantee that other parties will not mine our users' email addresses in any possible ways found over the Internet.
