Shoot induction on several cutting types of corm of shrimp banana (Musa acuminata Colla) through in vitro culture

Authors

  • Mayta Novaliza Isda Jurusan Biologi FMIPA Universitas Riau
  • Elvianis Elvianis
  • Siti Fatonah

DOI:

https://doi.org/10.25077/jbioua.8.1.20-28.2020

Abstract

Musa acuminata Colla is a unique genomic species which differs from other species in this genus. It has typical color of reddish-purple on its fruit. Musa acuminata is recorded as rare banana in Riau, particularly in Kampar District. This research aims to study the influence of several cutting types of corm of shrimp banana originated from Kampar District through in vitro culture. It also purposes to determine the best formation of M. acuminata shoot through various concentration treatments of BAP only and BAP combined with Kinetin. The study used randomized block design (RBD) by adding come BAP concentration (0, 4, 8 mg/l) and BAP combined with Kinetin (0 mg/l BAP+0.4 mg/l Kinetin, 4 mg/l BAP+0.4 mg/l Kinetin, and 8 mg/l BAP+ 0.4 mg/l Kinetin). The cutting type of banana corm involve no cut (whole) and half-to-half cutting in MS media, each with 5 replicates. The result showed that either addition of BAP and BAP combined with Kinetin gave the best percentage of live explants and shot formation up to 100%. Treatment of 8 mg/l BAP resulted in the highest shoot percentage up to 100%, the fastest shoots appearance at 34.00 days after planting and the longest shoot reached 2.83 cm with 2.33 shoots counted on half-to-half cutting type. This study also confirmed that the addition of plant regulation substances from cytokinin group gave good impact in the formation of shrimp banana shoots.

Author Biography

Mayta Novaliza Isda, Jurusan Biologi FMIPA Universitas Riau

Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Riau

References

Amelya, T. 2016. Respon Pembentukan Tunas Dengan Penambahan BAP dan Kinetin dari Eksplan Bonggol Pisang Tanduk (Musa corniculata Lour) secara in vitro. Prosiding Seminar Nasional Perhimpunan Pemuliaan Indonesia (PERIPI) Komda Riau “Strategi Pemuliaan dalam Mengantisipasi Perubahan Iklim Globalâ€Hal 77-82.

Arinaitwe, G., Rubaihayo, P.R., Magambo, M. J. S.2000. Proliferation Rate Effect of Cytokinin on Banana ( Musa spp) Cultivars.Sci.Hort.86:13-21

Armini, A.N. M., Wattimena., & Gunawan. L.W.1991. Perbanyakan Tanaman Bioteknologi Tanaman. Lab.Kultur Jaringan. .Bogor:Institut Pertanian Bogor.

Dhaliwal., & S, Harbinder. 2004. TIBA Inhibition of Invitro Organogenesis in Excised Tobacco Leaf Explants. In Vitro Cell. Dev. Biol. Plant 40:235-238

Gubbuk., Hamide., & Mustofa, P. 2004. In Vitro Propagation of Some New Banana Types (Musa spp.). Turk J Agric 28 (2004) 355-361.

Isda, M. N., & S, Fatonah. 2014. Induksi Akar pada Eksplan Tunas Anggrek Grammatophylum scriptum var. Citrinum secara In vitro pada Media MS dengan Penambahan BAP dan NAA. Jurnal Biologi Lingkungan. 7(2):53-52.

Isda, M.N., S, Fatonah., R, Y. Rahmawati. 2015. Induksi Tunas dari Eksplan Biji Manggis (Garcinia mangostana L.) Asal Bengkalis Secara In Vitro. Prosiding Semirata 2015 bidang MIPA BKS-PTN Barat. Universitas Tanjung pura Pontianak. Hal 166-172.

Kasutjianingati., & Boer, D. 2013. Mikropropagasi Pisang Mas Kirana (Musa acuminata L) Memanfaatkan BAP dan IAA secara In Vitro. Jurnal Agroteknos. 3(1):60-64.

Kuswandi, P.C. 2013. Pelatihan Kultur Jaringan Anggrek-Materi4:Bahan Tanaman (eksplan) dalam Metode Kultur Jaringan.Jurdik Biologi-FMIPA UNY.

Malik , T. A., Aish, M., Ahmed, Ch.MS., Quraishi .A. 2000. In Vitro Multiplication of Banana C.V. Desi. Pakistan Journal of Biological Sciences. 3(12) 2253-2255.

Noviana E. 2014. Induksi Tunas Pisang Rotan (Musa sp.(AA Group)) dari Eksplan Bonggol Anakan dan Meristem Bunga Secara In Vitro. Program Studi Agroteknologi Fakultas Pertanian dan Peternakan UIN SUSKA Riau. Pekanbaru.

Pierik, R. L. M. 1997. In Vitro Culture of Higher Plants. Martinus Nijhoff Publisher.Boxton.

Smith,R.H. 2000. Plant Tissue Culture. Technique and Experiments.Academic Press. California.

Surono, A. & A.Himawan. 2009. Perbanyakan Tiga Kultivar Pisang (Musa Paradisiaca L) Menggunakan Medium Murashige Skoog (MS) Instan dan Variasi Hormon Benzylaminopurin (BAP).Seminar Nasional Biologi XX dan Kongres Perhimpunan Biologi Indonesia XIV di Universitas Islam Negeri Maulana Malik Ibrahim Malang.

Wardani, P., Solichatun., Setyawan, A.D. 2004. Kultur Kalus Talinum paniculatum Gaertn. pada Variasi Penambahan Asam 2,4- Diklorofenoksi Asetat (2,4 D) dan Kinetin. Biofarmasi. 2(1):3-43

Wati, R,.S., M,.N, Isda., S. Fatonah. 2015. Induksi Tunas Dari Eksplan Bonggol Pisang Udang ( Musa acuminata Colla) Secara In Vitro Pada Media MS Dengan Penambahan BAP. Di dalam: Inovasi Eksplorasi Keanekaragaman Hayati Dan Konservasi Untuk Pembangunan Berkelanjutan. Prosiding Seminar Nasional Biodiversitas dan Ekologi Tropika Indonesia.

Wattimena, G.A. 1988. Zat Pengatur Tumbuh Tanaman. Lembaga Sumber Daya . Informasi IPB. Bogor.

Yusnita. 2004. Kultur Jaringan. Cara Memperbanyak Tanaman Secara Efisien. Jakarta: Agromedia Pustaka.

Yuwono. 2006. Bioteknologi Pertanian. Yogyakarta: Gadjah Mada University press.

Downloads

Published

2020-06-09

Issue

Vol. 8 No. 1 (2020)

Section

Articles

License

Please find the rights and licenses in Jurnal Biologi Universitas Andalas (JBioUA).

1. License

The non-commercial use of the article will be governed by the Creative Commons Attribution license as currently displayed on Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. 

2. Author’s Warranties

The author warrants that the article is original, written by stated author(s), has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).

3. User Rights

JBioUA's spirit is to disseminate articles published are as free as possible. Under the Creative Commons license, JBioUA permits users to copy, distribute, display, and perform the work for non-commercial purposes only. Users will also need to attribute authors and JBioUA on distributing works in the journal.

4. Rights of Authors

Authors retain the following rights:

  • Copyright, and other proprietary rights relating to the article, such as patent rights,
  • The right to use the substance of the article in future own works, including lectures and books,
  • The right to reproduce the article for own purposes, provided the copies are not offered for sale,
  • The right to self-archive the article,
  • the right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the article's published version (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal (Jurnal Biologi Universitas Andalas).

5. Co-Authorship

If the article was jointly prepared by other authors, they warrant that he/she has been authorized by all co-authors to sign this agreement on their behalf, and agrees to inform his/her co-authors of the terms of this agreement.

6. Royalties

This agreement entitles the author to no royalties or other fees. To such extent as legally permissible, the author waives his or her right to collect royalties relative to the article in respect of any use of the article by JBioUA or its sublicensee.

Privacy Statement

The names and email addresses entered in this journal site will be used exclusively for the stated purposes of this journal and will not be made available for any other purpose or to any other party.

However, due to the advancement and availability of hacking and data mining techniques found over the Internet, JBioUA will not be able to guarantee that other parties will not mine our users' email addresses in any possible ways found over the Internet.