The Effect of Centella (Centella asiatica (L.) Urb.) Extract with Several Types of Solvents as a Biostimulant on the Growth of Pagoda Mustard (Brassica rapa var. narinosa L.)

Authors

  • Jelita Putri Adisti Universitas Andalas
  • Suwirmen Suwirmen Departemen Biologi FMIPA Universitas Andalas
  • Muhammad Idris Departemen Biologi, FMIPA, Universitas Andalas

DOI:

https://doi.org/10.25077/jbioua.11.1.54-61.2023

Keywords:

plant physiology

Abstract

Biostimulants are natural organic compounds that can be applied to plants, so that they can modify plant physiology, stimulate growth and increase response to biotic and abiotic stresses. Biostimulants must have good solubility in suitable solvents. The research about the Effect of Centella (Centella asiatica (L.) Urb.) Extract with Several Types of Solvents as a Biostimulant on the Growth of Pagoda Mustard (Brassica rapa var. narinosa L.)  was conducted from March to June 2022 at the Plant Physiology Laboratory and Greenhouse, Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University, Padang. The research aims to determine the effect of C. asiatica extract with several types of solvents as a biostimulant and to determine the best type of solvent to extract C. asiatica as a biostimulant in increasing the growth of Pagoda mustard. This study used a completely randomized design (CRD) consisting of 5 treatments with 6 replications. The treatment consist of control, distilled water, methanol, ethanol and acetone. The results obtained in this study were C. asiatica extract with several types of solvents gave significantly different effects on the parameters of leaf number, leaf area and leaf chlorophyll content. The results of this study indicate that methanol and ethanol are the best solvents for making C. asiatica extract as a biostimulant that can increase the growth of Pagoda mustard.

References

Abbas, S. M. 2013. The Influence of Biostimulant on The Growth and on

The Biochemical Composition of Vicia faba CV. Giza 3 beans. Romanian Biotecnological Letters, 18(2): 8061-8068.

Agustina, J. 2014. Pengaruh Pupuk Urea Pupuk Organik Padat Dan Cair Kotoran Ayam Terhadap Sifat Tanah, Pertumbuhan Dan Hasil Kacang Tanah Di Tanah Inceptisol. Jurnal Penelitian Nusantara Cendana University, 2(1): 157-165.

Amriyanti, F.L dan Purity S.A. 2019. Aplikasi Sari Daun Kelor Sebagai Zat Pengatur Tumbuh Organik Terhadap Pertumbuhan dan Kadar Klorofil Tanaman Kedelai (Glycine max (L.) Merr.). Stigma. 12 (2): 82-88.

Astarina, N. G. H., K.W. Astuti dan N. K. Warditiani. 2013. Skrining Fitokimia Ekstrak Metanol Rimpang Bangle (Zingiber purpureum Roxb.). Jurnal Farmasi Udayana, 2(4): 29-37.

Amriyanti, F.L dan Purity S.A. 2019. Aplikasi Sari Daun Kelor Sebagai Zat Pengatur Tumbuh Organik Terhadap Pertumbuhan dan Kadar Klorofil Tanaman Kedelai (Glycine max (L.) Merr.). Stigma. 12 (2): 82-88.

Banks, J. M. and G. C. Percival. 2012. Evaluation of Biostimulants to Control Guignardia Leaf Blotch (Guignardia aesculi) of Horsechestnut and Black Spot (Diplocarpon rosae) of Roses. Arboriculture & Urban Forestry, 38(6): 258–261.

Banu H., I.C. Roberto dan O. Taolin. 2015. Pengaruh Dosis Pupuk Mitra Flora dan Ekstrak Daun Kelor (Moringa oleifera) Terhadap Pertumbuhan dan Hasil Tanaman Sawi (Brassica juncea, L.). Jurnal Pertanian Konservasi Lahan Kering, 1(1): 8-12.

Biradar, S.R. and B. D. Rachetti., 2013. Extraction of Some Secondary Metabolites & Thin Layer Chromatography from Different Parts of Centella asiatica L. (URB). American Journal of Life Sciences, 1(16): 243-247.

Bulgari, R.., G. Cocetta., A. Trivellini., P. Vernieri, and A. Ferrante. 2015. Biostimulants and crop responses: A review. Biological Agriculture and Horticulture. Taylor and Francis Ltd.

Cahyono, B. 2003. Teknik dan Strategi Budi Daya Sawi Hijau. Yayasan Pustaka Nusantara. Yogyakarta.

Calvo, P., L. Nelson and J.W. Kloepper. 2014. Agricultural Uses of Plant Biostimulants. Journal Plant and Soil 383: 3-41.

Do, Q. D., L. H. Huynh, A. E. Angkawijaya, P. L. Tran, Y. H. Ju, F. E. Soetaredjo and S. Ismadji. 2013. Effect of Extraction Solvent on Total Phenol Content, Total Flavonoid Content, and Antioxidant Activity of Limnophila aromatica. Journal of Food and Drug Analysis, 22(3): 296-302.

Drobek, M., F. Magdalena and C. Justyna. 2019. Plant Biostimulants: Importance of the Quality and Yield of Horticultural Crops and the Improvement of Plant Tolerance to Abiotic Stress A Review. Agronomy, 18(9): 335-342.

Du Jardin, P. 2012. The science of biostimulants, A Bibliography Analysis. Report On Biostimulant. April 2012.

Du Jardin, P. 2015. Plant Biostimulants: Definition, concept, main categories and regulation. Scientia Horticulturae, 196 : 3-14.

Fitri, D. A., W. Solichatun dan Mudyantini. 2004. Pengaruh Ekstrak Tanaman Kacang Hijau (Vigna radiata (L.) Wilczek.) terhadap Pertumbuhan dan Nodulasi Tanaman Kedelai (Glycine max (L.) Merr.). Jurnal BioSMART, 6(1): 24-28.

Guenther, E. 2006. Minyak Atsiri. Jilid IV B. Penerjemah S. Ketaren. Universitas Indonesia.

Harborne, J.B. 2006. Metode Fitokimia : Penuntun Cara Menganalisis Tumbuhan. diterjemahkan oleh Kosasih Padmawinata dan Iwang Soedira, Edisi kedua, 15-17, ITB, Bandung.

He, J.X., S. Fujioka, and T.C. Li. 2003. Sterols regulate development and gene expression in Arabidopsis. Plant Physiol. 131(3): 1258-1269.

Hendriyani, I.S., Y. Nurchayati, dan N. Setiari. 2018. Kandungan klorofil dan karotenoid kacang tunggak (Vigna unguiculata (L.) Walp.) pada umur tanaman yang berbeda. Jurnal Biologi Tropika, 1(2) : 38-43.

Hernani, T. Marwati dan C. Winarti. 2007. Pemilihan Pelarut Pada Pemurnian Ekstrak Lengkuas (Alpinia galanga) Secara Ekstraksi. Jurnal Pascapanen, 4(1): 1-8.

Huang, W., D. A Ratkowsky., C. Hui., P. Wang., J. Su and P. Shi. 2019. Leaf fresh weight versus dry weight: which is better for describing the scaling relationship between leaf biomass and leaf area for broad-leaved plants. Forests, 10(3): 256-274.

Kanwal, N., M. A. Randhawa and Z. Iqbal. 2017. Influence of processing methods and storage on physicochemical and antioxidant properties of guava jam. International Food Research Journal, 24(5): 2017-2027.

Mangera, Y. 2013. Analisis Pertumbuhan Tanaman Gandum Pada Beberapa Kerapatan Tanaman dan Imbangan Pupuk Nitrogen Anorganik. Jurnal Agricola, 3(2): 102-116.

Mattner, S.W., D. Wite, D.A. Riches, I.J. Porter, and T. Arioli,. 2013. The Effect of Kelp Extract on Seedling Establishment of Broccoli on Contrasting Soil Types in Southern Victoria, Australia. Biological Agriculture & Holticulture, 29(4): 258-270.

Mierziak, J., Kostyn, K., and Kulma, A. 2014. Flavonoids as Important Molecules of Plant Interactions with the Environment. Journal Molecules. 19(10): 16240– 16265.

Peer, W.A., A.S. Murphy. 2007. Flavonoid and Auxin Transport: Modulator or Regulators. Journal Elsivier Trends in Plant Science, 12 (12): 556-563.

Petrucci, H., S. Harwood., F. Herring dan D. Jeffrey. 2008. Kimia Larutan Edisi Kesembilan Jilid 2. Jakarta : Erlangga. Hal : 344.

Prasedya, E.S., B. A. Geraldine., N. N. Putri., A.S. Abidin., A. Jupri and H. Sunarpi. 2019. Effect of Solid and Liquid Extract of Sargassum crassifolium on Growth and Yield of Rice Plant. Jurnal Biologi Tropis, 20(3): 320-328.

Rahmadani, S. 2021. Pengaruh Ekstrak Kelor (Moringa oleifera L.) sebagai Biostimulan terhadap Pertumbuhan Kubis Singgalang (Brassica olerceae var. capitata L.). Skripsi Sarjana Biologi FMIPA Universitas Andalas. Padang

Reis, B., R. M Marques, H.A Silva., M. A Lolis., F.C Moreira., K. K Belato., dan C.M Bonato. 2011. High dilutions of acetone affect the Avena sativa growth in vitro. Int J High Dilution Res,10(36): 249-252.

Saha, S., T. Guria., T. Singha and T.K. Maity. 2013. Evaluation of analgesic and anti-inflammatory activity of chloroform and methanol extracts of Centella asiatica Linn. ISRN Pharmacol,

Salamah, N. dan E. Widyasari. 2015. Aktivitas antioksidan ekstrak metanol daun kelengkeng (Euphoria longan (L) Steud.) dengan metode penangkapan radikal 2,2’-difenil-1- pikrilhidrazil. Pharmaciana, 5(1): 25- 34.

Stewart, A. J., Chapman, W., Jenkins, G. I., Graham, I and Crozier, A. 2001. The effect of nitrogen and phosphorus deficiency on flavonol accumulation in plant tissues. Plant, Cell and Environment, 24: 1189 – 1197.

Suryani, N. C., D. Permana., M. gede dan A. A. Jambe. 2015. Pengaruh Jenis Pelarut Terhadap Kandungan Total FlavonoidEkstrak Daun Matoa (Pometia Pinnata). Jurnal Ilmu dan Teknologi Pangan, 5(1).

Suryaningrum, R.., E. Purwanto dan S. Sumiyati. 2016. Analisis Pertumbuhan Beberapa Varietas Kedelai pada Perbedaan Intensitas Cekaman Kekeringan. Agrosains: Jurnal Penelitian Agronomi. 18(2): 33-37.

Sutharsan, S., S. Nishanthi and S. Srikrishnah. 2014. Effects of Foliar Application of Seaweed (Sargassum crassifolium) Liquid Extract on the Performance of Lycopersicon esculentum Mill. In Sandy Regosol of Batticaloa District Sri Lanka. American-Eurasian J. Agric. & Environ, 14(12): 1386-1396.

Suwirmen, S., Z. A. Noli dan T. Rukmini. 2022. Aplikasi Ekstrak Padina minor dan Centella asiatica Sebagai Biostimulan Terhadap Pertumbuhan Tanaman Kedelai (Glycine max (L.) Merr.). Bioscientist : Jurnal Ilmiah Biologi, 10(1): 166-172.

Wientarsih, I., S. H. Sjarif dan I. M. Hamzah. 2013. Aktivitas Antioksidan Fraksi Metanol Daun Pegagan (Centella asiatica (L.) Urban). Fitofarmaka, 3(2): 2087-2091.

Zakiah, Z., I. Suliansyah., A. Bakhtiar and Mansyurdin. 2017. Effect of Crude Extracts of Six Plants on Vegetative Growth of Soybean (Glycine max Merr.). International Journal of Advances in Agricultural Science and Technology, 4(7): 1-12

Zatnika, I. 2010. Teknik dan strategi budidaya sawi hijau. Media Indonesia. Jakarta.

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Published

2023-03-15

How to Cite

Adisti, J. P., Suwirmen, S., & Idris, M. (2023). The Effect of Centella (Centella asiatica (L.) Urb.) Extract with Several Types of Solvents as a Biostimulant on the Growth of Pagoda Mustard (Brassica rapa var. narinosa L.). Jurnal Biologi UNAND, 11(1), 54–61. https://doi.org/10.25077/jbioua.11.1.54-61.2023

Issue

Vol. 11 No. 1 (2023)

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