Multiplikasi Tunas Tetrastigma rafflesiae Miq. pada Media Tanam Murashige-Skoog dengan Penambahan 6-Benzylaminopurine dan 1-Naphtaleneacetic Acid secara In Vitro

Authors

  • Ridha Permata Sari Laboratorium Fisiologi Tumbuhan, Jurusan Biologi, FMIPA, Universitas Andalas, Padang, Sumatera Barat 25163
  • - Suwirmen Laboratorium Fisiologi Tumbuhan, Jurusan Biologi, FMIPA, Universitas Andalas, Padang, Sumatera Barat 25163
  • M. Idris Laboratorium Fisiologi Tumbuhan, Jurusan Biologi, FMIPA, Universitas Andalas, Padang, Sumatera Barat 25163

DOI:

https://doi.org/10.25077/jbioua.2.4.%25p.2013

Abstract

The study about in vitro shoot multiplication of Tetrastigma rafflesiae miq. on murashige-skoog media with addition of 6-Benzylaminopurine and 1-Naphtaleneacetic Acid had purpose to determine physiological responses of Tetrastigma rafflesiae nodes with addition of 6-Benzylaminopurine (BAP) and 1-Naphtaleneacetic Acid (NAA) in several concentrations on shoot multiplication stages. This experiment used randomised complete design in 11 treatments and three replications. The treatment were the addition of BAP (1.0-5.0 mg/l) and its combination with NAA (0.1 mg/l) on MS medium. The results showed that nodus successfully producing shoot and callus. The addition of BAP was more effective than its combination with NAA in shoot multiplication. MS medium with addition of 2.0-4.0 mg / l BAP showed a better media for shoot multiplication (2.3-2.7 shoot each explant). Generally, MS media with combination of BAP and NAA produced a higher shoot although it did not significantly differed with BAP treatments. The result indicated that addition of BAP in in vitro medias have a high possibility of success for shoot multiplications in Rafflesia’s host plant conservation. Keywords: 1-Naphtaleneacetic Acid, 6-Benzylaminopurine, Murashige-Skoog media, Shoot multiplication.

References

Alizadeh, M., S. K. Singh and V. B. Patel. 2010. Comparative Performance of In Vitro Multiplication in Four Grape (Vitis sp.) Rootstock Genotypes. International Journal of Plant Production 4 (1) : 41-50.

Gunawan, L. W. 1988. Teknik Kultur Jaringan Tumbuhan. PAU Bioteknologi IPB. Bogor.

Idris, M. dan N. W. Surya. 2011. Inisiasi Tunas dari Potongan Hipokotil Tetrastigma leucostaphylum dalam Perbanyakan Inang Tumbuhan Rafflesia Secara In Vitro. Biospectrum 7 (1): 28-32.

Jiang, W., Y. Fu, X. Zhou, dan C. Fu. 2011. High-Frequency Shoot Regeneration of Nodal Explants from Tetrastigma hemsleyanum Diels et Gilg: A Valuable Medicinal Plant. African Journal of Biotechnology 10(57) : 12177-12181.

Lee, N. dan H. Y. Wetzstein. 1990. In Vitro Propagation of Muscadine Grape by Axillary Shoot Poliferation. J. Amer. Soc. Hort. Sci. 115(2): 324-329.

Mockaitis, K dan M. Estelle. 2008. Auxin Receptor and Plant Development: A New Signaling Paradigm. Annual Review of Cell and Developmental Biologi. 24: 55-80.

Osborne, D. J dan M. T. McManus. 2005. Hormones, Signals and Target Cells in Plant Development. Cambridge University. New York.

Ozden, M. dan M. Karaaslan. 2010. Effect of Cytokinin on Callus Proliferation Associated with Physiological and Biochemical Changes in Vitis vinifera L. Acta Physiol Plant. 33: 1451-1459.

Perilli, S., L. Moubayidin dan S. Sabatini. 2009. The Molecular Basis of Cytokinin Function. Plant Biology. 13: 21-26.

Sakakibara, H. 2006. Cytokinin: Activity, Biosyntesis and Translocation. Annual Review of Cell and Developmental Biologi. 57: 431-449

Stamp, J. A., S. M. Colby dan C. P. Maredith. 1990. Direct Shoot and Plant Regeneration from Leaves of Grape (Vitis spp.). Plant Cell, Tissue dan Organ Culture. 22: 127-133.

Sulastri, C. 2005. Kultur Tunas Pisang Kepok (Musa branchycarpa Backer.) pada Medium Murashige-Skoog dengan Penambahan BAP dan NAA. [Skripsi] Fakultas Matematika dan Ilmu Pengetahuan Alam. Universitas Andalas. Padang.

Surya, N. W. dan Idris M. 2011. A Preliminary Study on In vitro Seed Germination and Rooted Callus Formation of Tetrastigma rafflesiae (Vitaceae). Garden’s Bulletin Singapore 63(1 & 2): 507–513.

Veldkamp, J. F. 2007. The Correct Name for The Tetrastigma (Vitaceae) Host Of Rafflesia (Rafflesiaceae) In Malesia And A (Not So) New Species. Reinwardita 12(4): 261-265.

Zhang, P., Z. Y. Yu, Z. M. Cheng, Z. Zhang dan J. M. Tao. 2011. In Vitro Explant Regeneration of The Grape Wink (Vitis Vinifera L. Wink). Journal of Plant Breeding and Croop Science. 3(11): 276-282.

Zuhud E. A. M., Ekarelawan, Hikmat A. 1993. Bioekologi dan Penanggkaran Rafflesia rochusennii untuk Pelestarian Pemanfaatannya di Gunung Salak. Bogor: Fakultas Kehutanan Institut Pertanian Bogor. Bogor.

Zuhud, E. A. M., Hikmat A., dan Jamil N. 1998. Rafflesia Indonesia : Keanekaragaman, Ekologi dan Peletariannya. Jurusan Konservasi Sumberdaya Hutan – Fakultas Kehutanan Institut Pertanian Bogor.

Downloads

How to Cite

Sari, R. P., Suwirmen, .-., & Idris, M. (2015). Multiplikasi Tunas Tetrastigma rafflesiae Miq. pada Media Tanam Murashige-Skoog dengan Penambahan 6-Benzylaminopurine dan 1-Naphtaleneacetic Acid secara In Vitro. Jurnal Biologi UNAND, 2(4). https://doi.org/10.25077/jbioua.2.4.%p.2013

Issue

Vol. 2 No. 4 (2013)

Section

Articles

License

Please find the rights and licenses in Jurnal Biologi Universitas Andalas (JBioUA).

1. License
The non-commercial use of the article will be governed by the Creative Commons Attribution license as currently displayed on Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

2. Author's Warranties
The author warrants that the article is original, written by stated author(s), has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).

3. User Rights
JBioUA's spirit is to disseminate articles published are as free as possible. Under the Creative Commons license, JBioUA permits users to copy, distribute, display, and perform the work for non-commercial purposes only. Users will also need to attribute authors and JBioUA on distributing works in the journal.

4. Rights of Authors
Authors retain the following rights:
• Copyright, and other proprietary rights relating to the article, such as patent rights,
• The right to use the substance of the article in future own works, including lectures and books,
• The right to reproduce the article for own purposes, provided the copies are not offered for sale,
• The right to self-archive the article,
• the right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the article's published version (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal (Jurnal Biologi Universitas Andalas).

5. Co-Authorship
If the article was jointly prepared by other authors, they warrant that he/she has been authorized by all co-authors to sign this agreement on their behalf, and agrees to inform his/her co-authors of the terms of this agreement.


6. Royalties
This agreement entitles the author to no royalties or other fees. To such extent as legally permissible, the author waives his or her right to collect royalties relative to the article in respect of any use of the article by JBioUA or its sublicensee.


Privacy Statement
The names and email addresses entered in this journal site will be used exclusively for the stated purposes of this journal and will not be made available for any other purpose or to any other party.
However, due to the advancement and availability of hacking and data mining techniques found over the Internet, JBioUA will not be able to guarantee that other parties will not mine our users' email addresses in any possible ways found over the Internet.