Respon Pertumbuhan Nodus Artemisia vulgaris L pada Medium Murashige-Skoog dengan Penambahan Beberapa Zat Pengatur Tumbuh Secara In Vitro

Novia Rika Deli, Zozy Aneloi Noli, Suwirmen Suwirmen

Abstract

Artemesia vulgaris L produce artemisinin which is effective against resistant strains of Plasmodium falciparum, the malarial parasite. The study about growth responses of Artemesia vulgaris L in Murashige-Skoog (MS) medium supplemented with several growth regulators had been done from September to November 2014 at the Laboratory of Plant Phisiology and Tissue Culture, Department of Biology, Faculty of Mathematic and Natural Science, Andalas University. The aims of the study was to know the responses of node explants of Artemesia vulgaris L supplemented with several plant growth regulators for the effective technique of propagation. The study used Completely Randomized Design with four treatments and six replications. Node from in vitro growth were treated with three different kind of growth regulator (0.3 ppm BAP, 0.5 ppm NAA, 0.3 ppm GA3) in MS medium. The result showed that 0.5 ppm NAA was the best growth regulators formed callus, bud, and root of Artemesia vulgaris L.

Keywords: Artemesia vulgaris L, plant growths regulator, in vitro

Keywords

Artemesia vulgaris L.; plant growths regulator; in vitro

Full Text:

PDF

References

Abidin, Z. 1994. Dasar-Dasar Pengetahuan Tentang Zat Pengatur Tumbuh. Penerbit Angkasa. Bandung.

Badan POM RI. 2008. Taksonomi Koleksi Tanaman Obat, Kebun Tanaman Obat Citeureup. Badan Pengawas Obat dan Makanan Republik Indonesia Deputi Bidang Pengawasan Obat Tradisional, Kosmetik, dan Produk Komplemen Direktorat Obat Asli Indonesia. Jakarta.

Ferreira, J. F. S. and J. Janick. 1996. Distribution of Artemisinin in Artemisia annua. In J. Janick (Ed.). Progress in New Crops. ASHS Press. Arlington.

Fitriani, H. 2008. Kajian Konsentrasi BAP Dan NAA Terhadap Multiplikasi Tanaman Artemisia Annua L. Secara In vitro. Skripsi Fakultas Pertanian Universitas Sebelas Maret. Surakarta.

George, E. F. and Sherrington, 1984. Plant Propagation by Tissue Culture Handbook and Directory of Commercial Laboratories Exegetics Ltd. Erasley. Bassingtone. England. 551pp.

Gomez, K. A. dan A. A . Gomez.1995. Prosedur Statistik untuk Pertanian. Edisi Kedua. Universitas Indonesia. Jakarta.

Gulati, A. S . Bharel, S. K. Jain, M. Z. Abdin. and Srivastawa P.S. 1996. In vitro micropropagation and flowering in Artemisia annua. J. Plant Biochem. Biotechnol. 5: 31-35.

Gunawan, L. W. 1987. Teknik kultur jaringan. Laboratorium Kultur jaringan Pusat Antar Universitas (PAU) Bioteknologi IPB: Bogor

Hendaryono, D. P. S dan A. Wijayani, 1994. Teknik kultur jaringan, pengenalan dan petunjuk perbanyakan tanaman secara vegetatif modern. Kanisius. Yogyakarta.

Lestari, E, G. 2011. Peranan Zat Pengatur Tumbuh dalam Perbanyakan Tanaman melalui Kultur Jaringan. Jurnal AgroBiogen 7(1):63-68.

Liu C. Z., S. J. Murch, and M. El-Demerdash. (2003) Regeneration of Egyptian medicinal plant Artemisia judaica L. Plant Cell Rep. 21(6): 525-530.

Marlina, N dan D. Rusnandi. 2007. Teknik Aklimatisasi Planlet Anthurlum Pada Beberapa Media Tanam. Buletin Teknik Pertanian Vol. 12 No. 1.

Noggle, G. R and G. J. Fritz. 1979. Introductory Plant Physiologi. Second Edition. Perentice Hall of India Private LTD. New Delhi.

Nurhayati, H. dan Gusmaini. 2007. Potensi Pengembangan Budidaya Artemisia annua L. di Indonesia. Perspektif Vol. 6 No. 2 / Desember 2007. Hal 57 – 67 ISSN: 1412-8004.

Nursetiadi, E. 2008. Kajian Macam Media dan Konsentrasi BAP Terhadap Multiplikasi Tanaman Manggis (Garcinia Mangostana L.) Secara In Vitro. Skripsi Jurusan Agronomi Fakultas Pertanian Universitas Sebelas Maret Surakarta.

Prawiranata, W., H. Said, dan T. Pin. 1995. Dasar-dasar Fisiologi Tumbuhan. Jilid 2. Departemen Botani Fakultas Matematika dan IPA IPB: Bogor.

Santoso, U. dan F. Nursandi, 2004. Kultur Jaringan Tanaman. Universitas Muhammadiyah Malang Press. Malang.

Setiawati, W., R . Murtaningsih, N., Gunaeni, dan T. Rubiati. 2008. Tumbuhan Bahan Pestisida Nabati dan Cara Pembuatannya Untuk Pengendalian Organisme Pengganggu Tumbuhan (OPT). Prima Tani Balitsa ISBN: 978-979-8304-53-8. Balai Penelitian Tanaman Sayuran Pusat Penelitian dan Pengembangan Hortikultura Badan penelitian dan Pengembangan Pertanian. Bandung.

Wattimena, G. A. 1987. Zat Pengatur Tumbuh Tanaman. Pusat Antar Universitas Bioteknologi. Institut Pertanian Bogor. Bogor. 247 pp.

Yunita, R,. E. G. Lestari. 2008. Komunikasi Pendek. Perbanyakan Tanaman Artemisia Annua Secara In vitro. Jurnal Agrobiogen 4(1):41-44.

Yusnita. 1997. Pengaruh BAP, Medium Dasar dan Jenis Eksplan terhadap Pembentukan Tunas Melinjo (Gnetum gnemon L.) Secara In vitro. Jurnal grotopika. 2(2) : 29-3

DOI: https://doi.org/10.25077/jbioua.4.3.%25p.2015

Refbacks

  • There are currently no refbacks.