Induksi PLB Anggrek Vanda sumatrana Schltr. Liar Pada Media MS dengan Penambahan BAP dan NAA serta Ploidisasi dengan Kolkisin

Hanifah - Aini, Mansyurdin - Mansyurdin, Suwirmen - Suwirmen

Abstract


The study about PLB induction of wild Vanda sumatrana Schltr. on MS media suplement with BAP and NAA and ploidisation by colchicine treatment was conducted from December 2014 until November 2015 at the Laboratory of Genetics and Cell Biology and Laboratory of Plant Physiology and Tissue Culture, Biology department, Faculty of Mathematic and Natural Science, Andalas University, Padang. The study aimed to 1) knowing the best concentration of 6-Benzyl amino purin (BAP) and α-Naphtalene acetic acid (NAA) for Protocorm Like Bodies (PLB)  induction from shoot tip of V. sumatrana, 2) knowing the PLB response of V. sumatrana to concentrations and soak period of colchicine and 3) find the effective concentrations and soak period of colchicine to induce tetraploid on PLB of V. sumatrana. Shoot tips from in-vitro cultured of V. sumatrana  were subcultured on Murashinge and Skoog (MS) medium supplement with 3 mg/l BAP + 0,5 mg/l NAA, 3 mg/l BAP and 1,5 mg/l BAP. PLB of diploid V. sumatrana from the best treatment were soaked in 0.05% and 0.1% colchicine for 24 and 48 hours respectively in MS liquid medium, as control were set PLB without colchicine treatment. The results showed that MS medium supplemented with 1.5 mg/l BAP was the best formula to induce PLB. The highest percentage of survival rate of PLB and percentage of survived PLB regenerated shoot was obtained from 0.05% colchicine with 24 hours soak period treatment. The effective treatment to induce tetraploid on PLB of V. sumatrana Schltr. was obtained from 0.05% colchicine solution for 24 hours soak period.

Keywords


chromosome, colchicine, PLB, polyploidy, Vanda sumatrana

Full Text:

PDF

References


Atichart, P dan S. Bunnag. 2007. Polyploid induction in Dendrobium secundum (Bl.)Lindl. by in vitro techniques. Thai Journal of Agricultural Science., 40(1-2): 91-95.

Chaicharoen, S., A. Satrabhandhu dan M. Khuatrachue. 1995. In vitro induction of poliploidy in white mulberry (Morus alba var. s54) by colchicine treatment. J. Sci. Soc. Thailand. 21: 229-242.

Chulalaksananukul, W dan W. Chimnoi. 1999. Polyploid Induction in Centella asiatica (L.) Urban by Colchicine Treatment. J. Sci. Res. Chula. Univ. 24 (2): 55-65.

Colli, S dan G. B. Kerbauy. 1993. Direct root tip conversion of Catasetum into protocorm-like bodies. Effects of auxin and cytokinin. Plant Cell, Tissue and Organ Culture. 33: 39-44.

Comber, J. B. 2001. Orchids of Sumatra. The Royal Botanic Gardens. Kew.

Daryono, B. S. 1998. Pengaruh kolkisin terhadap pembentukan sel-sel melon tetraploid. Buletin Agro Industri, (5): 2 – 11.

Kabir, M. F., M. S. Rahman., A. Jamal., M. Rahman dan M. Khalekuzzaman. 2013. Multiple shoot regeneration in Dendrobium fimbriatum Hook An ornamental orchid. The Journal of Animal & Plant Sciences, 23(4): 1140-1145

Kamemoto, H., R. Sagarik dan S. Kasemsap. 1964. Chromosome numbers of sarcanthine orchid spesies of Thailand. Nat. Hist. Bull. Siam Soc. 20: 235-241.

Khosravi, A. R., M. A. Kadir., S. B. Kadzemin., F. Q. Zaman dan A. E. De Silva. 2009. RAPD analysis of colchicine induced variation of the Dendrobium Serdang beauty. African Journal of Biotechnology, 8(8): 1455-1465.

Luvina W.S, R. 2011. Induksi poliploidi pada anggrek Dendrobium strebloceras dengan kolkhisin. Universitas Brawijaya. Malang (Abstr).

Nurmalinda., S. Kartikaningrum., N. Q. Hayati dan D. Widyastoety. 2011. Preferensi konsumen terhadap anggrek Phlaenopsis, Vanda dan Dendrobium. J. Hort. 21(4): 372-384

Park, S. Y., E. C. Yeung., D. Chakrabarty dan K. Y. Paek. 2002. An efficient direct induction of protocorm-like bodies from leaf subepidermal cells of Doritaenopsis hybrid using thin-section culture. Plant Cell Rep. 21: 46-51.

Park, S. Y., H. N. Murthy dan K. Y. Paek. 2003. Protocorm-like body induction and subsequent plant regeneration from root tip cultures of Doritaenopsis. Plant Science. 164: 919-923.

Purwanto, A. W dan S. Endang. 2009. Pesona kecantikan anggrek vanda. Kasinus. Yogyakarta

Sarathum, S., M. Hegele., S. Tantiviwat dan M. Nanakorn. 2010. Effect of concentration and duration of colchicine treatment on polyploiy induction in Dendrobium scabrilingue L. Europ.J.Hort.Sci. 75 (3): 123-127.

Setyowati, M., E. Sulistyaningsih dan A. Purwanto. 2013. Induksi poliploidi pada kultur meristem batang bawang wakegi (Allium x wakegi Araki). Ilmu Petanian. 16(1); 58-76.

Sheelavanthmath, S. S., H. N. Murthy., B. P. Hema., E. J. Hahn dan K. Y. Paek. 2005. High frequency of protocorm like bodies (PLBs) induction and plant regeneration from protocorm and leaf sections of Aerides crispum. Scientia Horticulturae. 106: 395-401.

Silva, P. A. K. X. d. M. e., S. C. Jacques dan M. H. B. Zanettini. 2000. Induction and identification of polyploids in Cattleya intermedia LINDL. (Orchidaceae) by in vitro techniques. Ciencia Rural, Santa Maria, 30(1): 105-111.

Singh, R. J. 2003. Plant cytogenetics. Second Edition. CRC PRESS, Boca Raton, London, New York, Washington, D.C.

Sulistianingsih, R., Z.A. Suyanto dan E. N. Anggia. 2004. Peningkatan kualitas anggrek Dendrobium hibrida dengan pemberian kolkisin. Ilmu Pertanian, 11(1): 13-21.

Sun, Q., H. Sun., L. Li dan R. L. Bell. 2009. In vitro colchicine-induced polyploid plantlet production and regeneration from leaf explants of the diploid pear (Pyrus communis L.) cultivar, ‘Fertility’. Journal of Horticultural Science & Biotechnology, 84(5): 548–552.

Suryo. 1995. Sitogenetika. Gadjah Mada University Press. Yogyakarta.

Utami, D. S dan S. Hartati. 2012. Perbaikan genetik anggrek melalui persilangan intergenerik dan perbanyakan secara in vitro dalam mendukung perkembangan anggrek di Indonesia. Agrineca. 12(2).


Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

© Hak Cipta Jurusan Biologi Fakultas MIPA Universitas Andalas 

ISSN : 2303-2162