Bioaktivitas Antibakteri Lamun Thalassia hemprichii dan Enhalus acoroides

Arief Anthonius Purnama, Eti Meirina Brahmana


The objective of present study was to identify bioactivity of antibacterial Indonesian Seagrass Thalassia hemprichii dan Enhalus acoroides from Karang Tirta Beach, Padang City, West Sumatera. This is a preliminary research for a new alternative antibiotic from Seagrass in Indonesia. In this study the seagrass was examined by a maceration process and using the rotary evaporator to obtain viscous extract. The material for the bioactivity testing was performed upon several intended bacteria such as Escherichia coli, Stapillococcus aureus, and Bacillus subtilis. The bioactivity testing was performed by using the method of resazurin and MIC (Minimum Inhibitory Concentration), which is determined by looking at well blue-colored at the smallest concentration. The result revealed that the best antibacterial bioactivity of Thalassia hemprichii extract was a total of 62.50 μg/mL N-hexane solvent against Escherichia coli and Stapillococcus aureus bacteria. While the best antibacterial bioactivity of Enhalus acoroides extract is 15.62 μg/mL of N-hexane solvent against Stapillococcus aureus.


Antibacterial, Bioactivity, Enhalus acoroides, Thallasia hempricii.

Full Text:



Azwanida, N, N. 2015. A Review On The Extraction Methods Use In Medical Plants, Principle, Strength and Limitation. Med Aromatic Plants 4: 196-201.

Bwanga, F., Moses, J., Melles, H., Sven, H. 2010. Evaluation of seven tests for the rapid detection of multidrug- resistant tuberculosis in Uganda. Int. J. Tuberc. Lung Dis 14: 890–895.

Bjork, M., Fred, S., Elizabeth, M., Sven, B. 2008. Managing Seagrasses for Resilience to Climate Change. IUCN. Gland. Switzerland.

El Din., Nihal G., Shams., Zeinab M., El- Sherif. 2013 "Nutritional value of Cymodocea nodosa and Posidonia oceanica along the western Egyptian Mediterranean coast. " The Egyptian Journal of Aquatic Research 393: 153-165.

Green, P. E dan F. T, Short. 2003. World Atlas of Seagrasses. Prepared by the UIMEP World Conservation Monitoring Centre. University of California Press, Berkeley. USA.

Jawetz., Melnick., Adelberg’s., 2005 Medical Microbiology. Salemba Medika, Jakarta.

Kannan, R, R., Arumugam, R., Anantharaman, P. 2010. Antibacterial Potential Of Three Seagrasses Against Human Pathogens. Asian Pasific Journal Of Tropical Medicine: 890-893.

Kannan, R, R., Arumugam, R, Iyapparaj, P., Thangaradjou, T., Anantharaman, P. 2013. In Vitro Antibacterial, Cytotoxity and Haemolytic Activities and Phytochemical Analysis Of Seagrasses From The Gulf Of Mannar, South India. Food Chemistry 136: 1484-1489.

Kuo, J. 2007. New Monoecious Seagrass of Hallophila sulawesii (Hidrocharitaceae) from Indonesia. Aqauat Bot 87: 171-175.

Kuriandewa, T. E., Supriyadi I. H. 2006. Seagrass Mapping in East Bintan Coastal Area, Riau Archipelago, Indonesia, Indonesia. Coastal Marine Science 30 (1): 154-161.

Mani, A. E. Velammal, A dan Jamila, P. 2012. Phytochemicals of The Seagrass Syringodium Isoetifolium and Its Antibacterial And Insecticidal Activities, European Journal of Biological Sciences 4 (3): 63-67.

Martin, A., Camacho, M., Portaels, F., Palomino, J. C. 2003. Resazurin

microtiter assay plate testing of Mycobacterium tuberculosis susceptibilities to second-line drugs: rapid, simple, and inexpensive method. Antimicrob Agents Chemother 47 (11): 3616-3619.

Pandey, A., Shalini, T. 2014. Concept Of Standardization, Extraction and Pre Phytochemical Screening Strategies For Herbal Drug. Journal Of Pharmocognosy and Phytochemical 2 (5): 115-119.

Petrussa, E., Braidot, E., Zancani, M., Peresson, C., Bertolini, A., Patui, S., Vianello, A. 2013. Plant Flavonoids Biosynthessis, Transport and Involvement in Stress Responses. International Journal of Molecular Sciences 14 14950-14973.

Purnama, A. A., Zakaria, I. J., Nurdin, J. 2014. The Diversity And Distribution Of Seagrass In Karang Tirta Beach Padang City, West Sumatera. Prosiding International Conference Shiite 4th Annual Syiahkuala University (AIC -UNSYIAH) in conjunction With The 9th Annual International Workshop and Expo on Sumatra Tsunami Disaster and Recovery,

Universitas Syiah Kuala, Banda Aceh, 22 – 24 Desember 2014. Syiah Kuala University Press. pp: 172-176.

Rampersad, S.N. 2012. Multiple Applications of Alamar Blue as an Indicator of Metabolic Function and Celluar Health in Cell Viability Bioassays. J. Sensors: 12347-12360.

Rompas, R. A., Hosea, J. E., Adithya, Y. 2012. Isolation and Identification Of Flavonoids In Seagrass Leaf (Syringodium isoetifolium). PHARMACON 1(2): 222 - 227

Ravikumar, S., Thajuddin, N., Suganthi, P., Jacob Inbaneson, S., and Vinodkumar, T. 2010. Bioactive potential of seagrass bacteria against human bacterial pathogens. Journal of Environmental Biology 31(3): 387 - 392.

Ravikumar, S., Ali, M. S., Anandh, P., Ajmalkhan, M., and Dhinakaraj, M. 2011. Antibacterial activity of Cymodocea serrulata root extract against chosen poultry pathogens. Indian Journal of Science and Technology 4(2): 98-100.

Sani, R. N., Nisa, F. C., Andriani. R.D and Maligan, J. M. 2014. Yield Analysis and Phytochemical Screening Ethanol Extract of Marine Microalgae Tetraselmis Chui. Jurnal Pangan dan Agroindustri 2(2): 121-126.

Sarker, S. D., Lutfun, N., Yashodharan, K. 2007. Microtitre Plate-Based Antibacterial Assay Incorporating Resazurin as an Indicator of Cell Growth, and Its Application in the in Vitro Antibacterial Screening of Phytochemicals. Methods 42: 321 – 24.

Subhashini, P., Elangovan, D., Thirunavukkarasu, T., Jutta, P. 2013, Bioactive Natural Products From Marine Angiosperm: Abundance and Functions, Natural Product Bioprospect: 129 - 136.

Steele, L., Melanie, C., Anne, B., Tom, A. 2015. "Seagrass–pathogen interactions: ’pseudo induction’of turtlegrass phenolics near wasting disease lesions." Marine Ecology Progress Series 303: 123-131.

Umamaheswari, M., Asokkumar, K., Sivashanmugam , A. T., Remyaraju, A, Subhadradevi, V., Ravi, T.K. In vitro xanthine oxidase inhibitory activity of the fractions of Erythrina stricta Roxb. Journal of ethnopharmacology 124(3): 646-648.


  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

© Hak Cipta Jurusan Biologi Fakultas MIPA Universitas Andalas 

ISSN : 2303-2162