Isolasi dan Karakterisasi Parsial Bakteri Indigenous Pemfermentasi dari Buah Durian (Durio zibethinus Murr.)

Azizah Nurmalinda, - Periadnadi, - Nurmiati

Abstract

The research related to the isolation and partial characterization of indigenous fermenting bacteria from durian fruit (Durio zibethinus Murr.) was conducted from May to August 2012 in Laboratory of Microbiology, Biology Department, Andalas University. Experimental method was used and was descriptively analyzed. Ripe durian fruit contain indigenous fermenting as well as nonfermenting bacteria, that was recognized from halozone around the colony on modified GTA medium. Indigenous fermenting bacterias on the flesh of durian fruit have been characterized as Gram-positive, bacil, no endospores, motile and catalase positive. These isolates are proteolytics, amylolytics and cellulolytics and have no ability to hydrolyze fats and alcohol.


Keywords : durian, fermenting, indigenous bacteria

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References

Akhdiya, A. 2003. Isolasi Bakteri Penghasil Enzim Protease Alkalin Termostabil. Buletin Plasma Nutfah 9 (2): 38-44.

Bevilacqua, A.E and A.N, Califano. 1989. Determination of organic Acid in Dairy Product by High Performance Liquid Chromatography. J. Food Sci 56 (4): 1076-1077.

Fardiaz, S. 1993. Analisis Mikrobiologi Pangan. Raja Grafindo Persada. Jakarta.

Fatoni, A., Zusfahair dan P. Lestari. 2008. Isolasi dan Karakterisasi Protease Ekstraseluler dari Bakteri dalam Limbah Cair Tahu. Natur Indonesia 10 (2): 83-88.

Huang, G., Ying T, Huo P & Jiang J. 2006. Purification and Characterization of A Protease from Thermophilic Bacillus strain HS08. African. Biotechnol 5: 2433-2438.

Jamilah, I., A. Meryandini, I. Rusmana, A. Suwanto dan N. R. Mubarik. 2009. Activity Proteolytic and Amylolitic Enzymes From Bacillus spp. Isolated from Shrimp Ponds. Journal Microbiology Indonesia 3 (2): 67-71.

Jamilah, I. 2011. Penapisan Bacillus dan Karakterisasi Protease dan Amilase Ekstraseluler yang Dihasilkan untuk Degradasi Sisa Pakan Pada Budi Daya Udang.Tesis. Jawa Barat. Institut Pertanian Bogor.

Kouker, G and K. E. Jaeger. 1987. Spesific and Sensitive Plate Assay for Bacteri Lipases. Applied and Enviromental Microbiology 53: 211-213.

Labeda D. P. 1990. Environmental Biotechnology. Isolation of Biotechnological Organisme From Nature. Mc. Graw Hill Publishing Company. USA.

Meryandini, A., W. Widosari, B. Maranatha, T.C. Sunarti, N. Rachmania dan H. Satria. 2009. Isolasi Bakteri Selulolitik dan Karakterisasi Enzimnya. Makara Sains 13(1): 33-38.

Pakpahan, R. 2009. Isolasi Bakteri dan Uji Aktivitas Protease Termofilik dari Sumber Air Panas Sipoholon Tapanuli Utara Sumatera Utara.Tesis. Medan. Universitas Sumatera Utara.

Periadnadi. 2003. Vorkommen und Stoffwechsellistungen von Bakterien der Gattungen Acetobacter und Gluconobacter Währen der Weinbereitung unter Berücksichtigung des Zucker_Säure_Stoffwechsels. Disertasi. Johann Wolfgang Goethe-Universität, Frankfrut aM.

Putri, W. D. R., Haryadi., D. W. Marseno dan M. N. Cahyanto. 2012. Isolation and Characterization of Amylolytic Lactic Acid Bacteria during Growol Fermentation, an Indonesian Traditional Food. Teknologi Pertanian 13(1): 52-60.

Rahmadani, S. Y. 2011. Keberadaan dan Karakteristik Mikroflora Indigenous Pulp Tiga Varietas Kakao (Theobroma cacao, L.) Di Sumatera Barat. Skripsi. Padang. Universitas Andalas.

Schlegel, H.G. dan K. Schmidt. 1994. Mikrobiologi Umum Ed. Ke-6. Gadjah Mada University Press. Yogyakarta.

Yuliana, N. 2005. Identifikasi Bakteri Bukan Penghasil Asam Laktat yang Berasosiasi dengan Tempoyak (Fermented Durian). Mikrobiologi Indonesia 10 (1): 25-28.

Yuliana, N. 2007. Pengolahan Durian (Durio zibethinus) Fermentasi (Tempoyak). Teknologi dan Industri Hasil Pertanian 12 (2): 70-80.

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