Karakterisasi Bakteri Termofilik Penghasil Enzim Protease Netral

Widia Firliani, Anthoni Agustien, Fuji Astuti Febria

Abstract

A study on the characterization of thermophilic bacteria in producing neutral protease enzyme has been conducted in the Laboratory of Microbiology, Biology Department, Andalas University from October 2013 to March 2014. The bacteria were isolated from a hot spring at Sungai Medang, Kerinci. Water samples  were collected purposively based on gradient temperature at the hot spring which ranged from 450C to 880C. Colony of the bacteria were inoculated using nutrient agar (NA). We obtained 10 isolates of neutral protease enzyme from the thermophilic bacteria with proteolytic index ranged from 0.27 to 3.89. The MIV-14 isolate has the highest proteolytic index which collected from 830C of the hot spring. Characterization of macroscopic, microscopic and biochemical tests were reported. Generally, the colonies have round shape, wavy edge, white, producing endospores and motile.

 

Keywords: characterization, neutral protease, thermophilic bacteria.

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References

Agustien, A. 2010. Protease Bakteri Termofilik. UNPAD PRESS. Bandung.

Atlas, R.M and Ronald, 1997. Principles of Microbiology, The 2nd Ed., WBC Mc Grow-Hill Book New York.

Benerjee, U.C., R.K. Sani., W. Azmi, and R. Soni. 1999. Thermostable Alkaline Proteases from Bacillus brevis and its Characterization as a Laundry Detergent Additive. Prosess Biochemistry, 35: 213-219.

Dirnawan, H., A. Suwanto, dan T. Purwadaria. 2000. Eksplorasi Bakteri Penghasil Enzim Hidrolitik Ekstraselluler dari Sumber Air Panas Gunung Pancar. Hayati, 7: 52-55.

Kumar, S. And R. Nussinov. 2001. How do Thermophilic Protein deals with heat? A review. Cell molecular life science, 58: 1216-1233.

Kurniawan, H.M. 2011. Isolasi dan Optimasi Ekstrinsik Bakteri Termo-proteolitik Isolat Sumber Air Panas Semurup Kabupaten Kerinci, Jambi. Tidak dipublikasikan. Pasca Sarjana Universitas Andalas. Padang.

Madigan, M.T.J., M. Martinko, and J. Parker. 2000. Biology of Microorganisms. The 9th Ed. Prentice Hall International, Inc. New Jersey.

Rahayu, U. 2007. Skrining Bakteri Termofilik Penghasil Protease, Buletin Teknisi Litkayasa Akuakultur, 6, 2: 146.

Rao, M.B., A.M. Tanksale, M.S. Gahtge, and V.V, Deshpande. 1998. Molecular and Biotechnological Aspects of Microbial Protease. Microbiology Biology Review, 62: 597-635.

Suhartono, M.T. 1991. Protease, PAU Bioteknologi IPB. Bogor.

Wahyuna, D. 2012. Isolasi dan Karakterisasi Bakteri Termo-Proteolitik dari Sumber Air panas Sungai Medang. Universitas Andalas. Skiripsi Sarjana Universitas Andalas. Padang.

Ward, O. P and Fogarty W. M, 1983. Proteinase. Microbial Enzyme and Biotechnology. Applied Science Publisher. New York

DOI: https://doi.org/10.25077/jbioua.4.1.%25p.2015

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