Penapisan dan Karakterisasi Bakteri Amilo-Termofilik dari Sumber Air Panas Semurup, Kerinci, Jambi

Wira Hastuti, Anthoni Agustien, - Nurmiati

Abstract

The screening and characterization of amylo-thermophylic bacteria that produced amylase from Semurup hot spring in Kerinci, Jambi was conducted from May to July 2012. The aim of this study was to find out the isolate of amylo-thermophylic bacteria and to analyze amylase activities. This study used experimental methods. This study found 31 isolates of amylo-thermophylic bacteria with the amylolytic index more than 2. The isolate SII- 1.7 has the highest enzyme activity. The form of colony was circular margin, smooth shiny surface and pigmentation cream. The bacterium was gram negative, bacilli cell, positive catalase, non-motile, and has proteolytic and amylolytic activities.


Keywords: amylolytic, bacterium, characterization, hot spring, thermophylic

Full Text:

PDF

References

Agustien, A. 2010. Protease Bakteri Termofilik. UNPAD PRESS. Bandung.

Atlas, R.M. 1997. Handbook of Microbological Media, 2nd edition. CRC Press. London.

Cappucino, J.G and N. Sherman. 1983. Microbiology: A Laboratory Manual. Addison Wesley Publishing Company. New York.

Cluskey, J.E., C.A. Knutson dan G.E. Inglett, 1980, Fractionation and characterization of dent corn and amylomaize starch granules. Journal Starch. 32 (4): 105.

Darwis, A.A. dan Sukara, E. 1990. Isolasi, purifikasi dan karakterisasi enzirn. Departemen P & K. Dirjen Pendidikan Tinggi. PAU Bioteknologi, Institut Pertanian Bogor. Bogor.

Falch, E. A. 1991. Industrial enzymes-developments in production and application. Journal Biotechnology Advances. 9 : 643-658.

Greenwood, C.T. and D.N. Munro. 1979. Carbohydrates. Applied Science Publ. Ltd., London.

Kaur, A. M. Kaur, L. Manohar and A. Zabeer. 2012. Isolation, characterization and identification of bacterial strain producing amylase. Journal Microbiology Technology. 2 (4):573-579

Leveque, E., S. Janecek, B. Haye, and A. Belarbi. 2000. Thermofilik Archaeal Amilolitik Enzymes. Enzyme and Microbial Technology. 26: 3 – 14

Madigan, M.T, J.M. Martinko and J. Parker. 2000. Brock Biology of Microorganism. Prentice Hall. New Jersey.

Margaretha, M. 2003. Penapisan Dan Karakterisasi Sejumlah Isolate Bakteri Termofilik Amilolitik .[Skripsi]. Institut Pertanian Bogor. Bogor

Richana, N, G.M. Yusuf, P. Lestari dan D.S. Damardjati, 1999. Perilaku Kultivasi Isolat Bakteri Termofil Penghasil α – amylase. Jurnal Mikrobiologi Indonesia . 4 (2): 35 – 39

Sumantha,A., C. Laroche and A. Pandey. 2006. Microbiology and industrial of food grade proteases : A perspective. Food Technology Biotechnology, 44 (2):211-220

Uhling, H. 1998. Industrial Enzymes and Their Applications. Academic press. New York.

DOI: https://doi.org/10.25077/jbioua.1.2.%25p.2012

Refbacks

  • There are currently no refbacks.