Paku Kawat Lycopodiella cernua (L.) Pic. Serm. (Lycopdiaceae-Lycopodiales) dari Provinsi Riau – Kajian Morfologi dan Sekuen DNA berdasarkan Primer RBCL

Nery Sofiyanti, Mayta Novaliza Isda

Abstract

Moss club (Lycopodiella cernua) is one of fern members distributed in Riau Province. The aimed of this study were to characterized the morphological characters and to analized its DNA sequence based on rbcl primer. Samples were collected from the field, documented and characterized the morphological charaters. DNA were isolated and amplified using PCR method and rbcl primer.  DNA sequnces were then analized using BLAST   Lycopodiella cernua collected from Riau Province was characterized by having creeping and erect rhizome that covered by microphyl, and terminal strobili. Rbcl fragmen of Lycopodiella cernua was sucssesfully amplified with 643 bp length. BLAST result confirmed the  rbcl fragmen of this club moss.

Keywords

club moss, DNA, morphology, rbcl

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References

Bafeel, S.O, Arif, I.A., Baki, M.A., Al Homaidan, A.A,, Al Farhan, A.H., Khan, H.A. 2012. DNA barcoding of arid wild plants using rbcL gene sequences. Genet Mol Res. 11(3):1934-41. doi: 10.4238/2012.July.19.12

Chuang, S. & Hu, J. 2004 The Evolution of Chloroplast matK Genes, Including Identification of New Homologues from Ophioglossum petiolatum and Two Lycophytes Taiwania, 49(4): 273-287

Dubuisson, J., Hennequin, S., Douzery, E.J.P., Cranfill, R.B., Smith, A.R., & Pryer, K.M. 2003. Rbcl Phylogeny Of The Fern Genus Trichomanes (Hymenophyllaceae), With Special Reference To Neotropical Taxa. Int. J. Plant Sci. 164(5):753–761.

Ebihara, A.. 2011. RbcL Phylogeny of Japanese Pteridophyte Flora and Implications on Infrafamilial Systematics Bull. Natl. Mus. Nat. Sci., Ser. B, 37(2), pp. 63–74, May 22, 2011

Evans, S. & Warnow, T. 2018. 2Phylogenetic analyses of alignments with gaps . https://statistics.berkeley.edu/sites/default/files/tech-reports/807.pdf

Gielly, L. & Taberlet, P. 1994. The Use of Chloroplast DNA to Resolve Plant Phylogenies: Noncoding versus rbcL Sequences Mol. Biol. Evol. 11(5):769-777.

Hasebe, M. 1995. Fern Phylogeny Based on rbcl Nucleotide Sequence. American Fern Journal 85(4): 134 – 181.

NCBI.2008. https://ncbi.nlm.nih.gov.

Sofiyanti N, D. Iriani, A.A. Roza. 2015a. Morfologi tumbuhan Paku di Taman Hutan Raya Sultan Syarif Hasyim, Riau. Unri Press, Pekanbaru.

Sofiyanti, N. & Isda, MN. 2017. Pengembangan Metode SEM dan analisis DNA dalam sistematika tumbuhan paku. Laporan PBK tahun I (Tidak dipublikasikan).

Sofiyanti, N. & Isda, MN. 2018. Pengembangan Metode SEM dan analisis DNA dalam sistematika tumbuhan paku. Laporan PBK tahun II (Tidak dipublikasikan).

Wikstrom, N. & Kenrick, P. 2000. Relationships of Lycopodium and Lycopodiella Based on Combined Plastid rbcL Gene and trnL Intron Sequence Data Systematic Botany 25(3): 495-510

Wikstrom, N. & Kenrick, P. 2001. Evolution of Lycopodiaceae (Lycopsida): Estimating Divergence Times from rbcL Gene Sequences by Use of Nonparametric Rate Smoothing. Molecular Phylogenetics and Evolution 19(2): 177-186. https://doi.org/10.1006/mpev.2001.0936.

Wikstrom, N. & Kenrick, P. 2008. Phylogeny of epiphytic Huperzia (Lycopodiaceae): paleotropical and neotropical clades corroborated by rbcL sequences. Nordic Journal of Botany. https://doi.org/10.1111/j.1756-1051.2000.tb01561.x

Zhang, L.B. & Iwatsuki, K. 2013. Lycopodiaceae. Pp. 13–34 in Z. Y. Wu, P. H. Raven & D. Y. Hong, eds., Flora of China, Vol. 2–3 (Pteridophytes). Beijing: Science Press; St. Louis: Missouri Botanical Garden Press.

DOI: https://doi.org/10.25077/jbioua.7.1.43-50.2019

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