Antifungal Activity of Endophytic Bacteria isolated from Pegagan (Centella asiatica L.) for Inhibition the Growth of Malassezia furfur

Vivi Yanthi, Mahyarudin Mahyarudin, Ambar Rialita

Abstract

Pityriasis versicolor is a superficial fungal infection characterized by changes in skin pigment due to stratum corneum colonization by Malassezia furfur. The Increasing of antifungal resistance and high recurrence rate, requires alternative treatment of medicinal plants. Pegagan (Centella asiatica L.) is known to produce secondary metabolites that have antifungal activity produced by endophytic bacteria that live on pegagan plant tissue. The study aimed to determine the antifungal activity of pegagan endophytic bacteria isolates to Inhibit the Growth of Malassezia furfur. The endophytic bacteria isolates were re cultured on NA media with a streak plate method. Antifungal activity test used the disk diffusion method which is characterized by the formation of inhibitory zones. Total of 37 endophytic bacteria isolates were successed re cultured, and there were 15 endophytic bacterial isolates that have potentcy as antifungal agent with inhibitory zones ranged from 6.5 to 15.52 mm. The most potential isolate was similarity with genus Pseudomonas. The secondary metabolites contained of alkaloids, terpenoids and saponins. The potential Centella asiatica endophytic bacteria had antifungal activity and similarity with the genus Pseudomonas.

Keywords

Endophytic bacteria; Antifungal activity,;Centella asiatica L.; Secondary metabolite,;Malassezia furfur;

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References

Adityawarman. 2017. Isolasi, Identifikasi dan Aktivitas Antibakteri Bakteri Endofit Daun Pegagan (Centella asiatica L.) terhadap Escherichia coli.[Skripsi]. Pontianak. Universitas Tanjungpura.

Afriani, A. 2018. Isolasi Bakteri Endofit Asal Tanaman Tebu dan Potensinya Sebagai Agens Biokontrol dan Pemacu Pertumbuhan, Langsa. J Penelitian. Agro Samudra. 4(2).

Alfiah, R.R., S. Khotimah, dan M. Turnip. 2015. Efektivitas Ekstrak Metanol Daun Sembung Rambat (Mikania micrantha Kunth) terhadap Pertumbuhan Jamur Candida albicans, Pontianak. Jurnal Protobiont 4(1): p 53.

Baker, S., and S. Satish. 2013. Bioprospecting of Endophytic Bacterial Plethora from Medicinal Plant. Plant Sciences Feed 3: 42-5.

Chen, S.C.A., and T.C. Sorrell. 2007. Antifungal Agents. Med J Aust 187(7):404-9.

Dinas Kesehatan Kota Pontianak. 2015. Prevalensi Kejadian Penyakit Jamur Akibat Infeksi. Departemen Kesehatan. Pontianak.

Effendi, M. 2013. Pemanfaatan Sistem Pengobatan Tradisional (Battra) di Puskesmas.[Skripsi]. Surabaya. FISP-UNAIR.

Faisal, I.A., M. Handini, and Mahyarudin. 2018. Aktivitas Quorum Quenching Bakteri Gram Positif Endofit Tanaman Pegagan (Centella asiatica) terhadap Chromobacterium violaceum.[Skripsi]. Pontianak. Fakultas Kedokteran Universitas Tanjungpura.

Freiesleben, S.H., and A.K. Jäger. 2014. Correlation between Plant Secondary Metabolites and Their Antifungal Mechanisms? A Review. Med Aromat Plants 154 (3).

Gaitanis, G., P. Magiatis, M. Hantschke, I.D. Bassukas, and A. Valegraki. 2012. The Malassezia Genus in Skin and Systemic Disease. Clin Microbiol Rev 25(1):106-141.

Han, A., D.A. Calcara, W.V. Stoecker, J. Daly, D.M. Siegel, and A. Shell. 2009. Evoked Scale Sign of Tinea versicolor. Arch Dermatol 145(9): 1078.

Hidayat, M., Mufidah, and R. Rante. 2018. Isolasi dan Karakterisasi Molekuler Mikroba Endofit Tanaman Pegagan (Centella asiatica L.) sebagai Penghasil Antimikroba. MFF 22(2):56-60.

Holt, J.G.. 2000. Bergey’s of determinative bacteriology. 9th edition. A Wolters Kluwer Company Philadelphia. USA. P: 471–473.

Jorgensen, J.H., and M.A. Pfaller. 2017. Manual of Cclinical Mmicrobiology. 11th edition. Washington DC.

Khrisnamurti, A.. 2014. Tingkat Pengetahuan Siswa SMAN 1 Semarang tentang Hygiene Personal terhadap Penyakit Panu.[Skripsi]. Semarang. Universitas Diponegoro.

Miller, K.I., C. Qing, D.M. Sze, B.D. Roufogalis, and B.A. Neilan. 2012. Culturable Endophytes of Medicinal Plants and the Genetic Basis for their Bioactivity. Microbial Ecology 64: 431-449.

Milliana, A., and W. Safitri. 2015. Isolasi dan Identifikasi Bakteri Endofit Rimpang Temulawak (Curcuma xanthorrhiza) sebagai Penghasil Senyawa Antifungi terhadap Candida albicans. El-Hayah 5(2):49–63.

Prayitno, H. 2015. Uji Aktivitas Antifungal Ekstrak Metanol Mentah Rimpang Jeringau Merah (Acorus calamus linn.) terhadap Pertumbuhan Malassezia furfur Secara In Vitro.[Skripsi]. Pontianak. Fak Kedokteran Univ Tanjungpura.

Radisu, A.S.. 2012. Distribusi Kejadian Tinea versicolor pada Anak Sekolah Dasar (SDN) 53 Sungai Raya Kabupaten Kubu Raya Berdasarkan Karakteristik dan Faktor Resiko [Skripsi]. Pontianak. Universitas Tanjungpura.

Strobel, G.A., and B. Daisy. 2003. Bioprocessing For Microbial Endophytes And Their Natural Products. Microbiology and Molecular Biology Reviews 67(4):491-502.

Strobel, G.A., and B. Daisy. 2004. Castillo U, Harper J. Natural products from endophytic microorganisms. Journal of Natural Products 67(2): 257-268.

Taechowisan, T.,C. Lu, Y. Shen, and S Lumyong. 2005. Secondary metabolites from endophytic streptomyces aureofaciens CMUAc130 and their antifungal activity. Microbiology 151:1691-1695.

Usatine, R.P. 2009. Tinea versicolor. McGraw Hill Companies. New York. P: 566-569.

Winarto, W.R., and M. Surbakti. 2003. Khasiat dan Manfaat Pegagan. Agromedia Pustaka. Jakarta.

Yuliar, Suciatmih, and D. Supriyati. 2013. Biodiversity Of Endophytic Bacteria And Their Antagonistic Activity To Rhizoctonia Solani And Fusarium qxysporium. Global Journal of Biology, Agriculture & Health Science 2(4):111-118.

Zearah, S.A.. 2014. Antifungal and Antibacterial Activity of Flavonoid Extract From Terminalia chebula Retz. Fruits. J Basrah Res 40(1):122–131.

Zhukov, V.A., O.Y. Shtark, A.Y. Borisov, and I.A. Tikhonovich. 2013. Breeding to Improve Symbiotic Effectiveness of Legumes.Croatia. Rijeka: 167–207.

DOI: https://doi.org/10.25077/jbioua.10.1.23-32.2022

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