Complementary Molecular Data for Two Species from Herbarium Bogoriense: Sphaerobolus stellatus and Trichaleurina javanica

Meta Yuliana, Indra Maulana, Sari Mulya, Laifa Fusvita, Rudy Hermawan

Abstract

A micro-mushroom namely Sphaerobolus stellatus (BO 24422) and macro-mushroom namely Trichaleurina javanica (BO 24420) were found in Landscape Arboretum of IPB University. The latest identification used morphological characteristic and molecular study of Large Subunit DNA marker respectively. According to their identification, the database of fungal identification is commonly from Internal Transcribed Spacer (ITS) marker for molecular study. But both specimens, S. stellatus BO 24422 and T. javanica BO 24420, have not the ITS sequences yet. This research aim to study the molecular data of two specimens using ITS sequences. The method of this research used the molecular identification using internal transcribed spacer (ITS) primer: ITS 4 as a forward and ITS 5 as a reverse. Two phylogenetic trees were generated by Randomized Axelarated Maximum Likelihood (RAxML) Black box. Bootstrap (BS) values ≥ 90% were displayed on the phylogenetic treebranches. The result showed that the bootstrap value for S. stellatus BO 24422  showed the good value as 96% classified as S. stellatus. Then, T. javanica BO 24420  had a better BS value with 93% classified as T. javanica. The ITS is a general marker and very useful for molecular identification.

 

Full Text:

PDF

References

Baetsen-Young, A. M., Kaminski, J. E., Kasson, M. T., & Davis, D. D. (2015). Insights into the biology of sphaerobolus stellatus as a causal agent of thatch collapse in golf turfs. Crop Science, 55(5): 2342–2351. https://doi.org/10.2135/cropsci2014.12.0821.

Carbone, M., Yei-Zeng, W., Chun-Lin, H. 2013. Studies in Trichaleurina (Pezizales).Type studies of Trichaleurina polytricha and Urnula philippinarum. The status ofSarcosoma javanicum, Bulgaria celebica, and Trichaleurina tenuispora sp. nov., withnotes on the anamorphic genus Kumanasamuha. Ascomycete.org., 5, 137-153.

Geml, J., Davis, D. D., Geiser, D. M. 2005. Phylogenetic analyses reveal deeply divergent species lineages in the genus Sphaerobolus (Phallales: Basidiomycota). Molecular Phylogenetics and Evolution, 35(2), 313–322. https://doi.org/10.1016/j.ympev.2005.01.014.

Hermawan, R., Maulana, I. 2020. Sphaerobolus stellatus: Cannonball Mushroom from West Java. Jurnal Mikologi Indonesia, 4(2),218–222. https://doi.org/10.46638/jmi.v4i2.86.

Hermawan, R., Amelya, M. P., & Julia, Z. R. 2020. Trichaleurina javanica from West Java. Jurnal Mikologi Indonesia, 4(2), 175–181. https://doi.org/10.46638/jmi.v4i2.85.

Hermawan, R., Maulana, I., Khairillah, Y. N. 2022. The first record of Nectriopsis rexiana Carried in Sphaerobolusstellatus Mycelium. Bioeduscience Journal, 6(2): 181-187. https://doi.org/10.22236/j.bes/628955.

Jeppson, M., Nilsson, R. H., Larsson, E. 2013. European earthstars in Geastraceae (Geastrales, Phallomycetidae)-a systematic approach using morphology and molecular sequence data. Systematics and Biodiversity, 11 (4): 437-465. http://dx.doi.org/10.1080/14772000.2013.857367.

Lucking, R., Aime, M. C., Robbertse, B., Miller, A. N., Ariyawansa, H. A., Aoki, T., Cardinali, G., Vrous, P. W., Druzhinina, I. S., Geiser, D. M., Hawksworth, D. L., Hyde, K. D., Irinyi, L., Jeewon, R., Johnston, P. R., Kirk, P. M., Malosso, E., May, T. W., Meyer, W., Opik, M., Robert, V., Stadler, M., Thines, M., Vu, D., Yurkov, A. M., Zhang, N., Schoch, C. L. 2020. Unambigous identification of fungi: where do we stand and how accurate and precise is fungal DNA barcoding?. IMA Fungus, 11(14): 1-32. http://doi.org/10.1186/s43008-020-00033-z.

Schoch, C. L., Seifert, K. A., Huhndorf, S., Robert, V., Spouge, J. L., Levesque, C. A., & Chen, W. (2012). Nuclear ribosomal internaltranscribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of The National Academy of Sciencesof The United States of America, 109(16), 6241–6246. https://doi.org/10.1073/pnas.1117018109.

Stamatakis, A. 2014. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30(9): 1312–1313. https://doi.org/10.1093/bioinformatics/btu033.

The Global Fungal Red List Initiative. 2015. Sphaerobolus stellatus. http://iucn.ekoo.se/iucn/species_view/196509/ (accessed 28 August 2023).

DOI: https://doi.org/10.25077/jbioua.12.1.42-46.2024

Refbacks

  • There are currently no refbacks.