Analisis Histologis Ginjal Ikan Asang (Osteochilus hasseltii ) di Danau Maninjau dan Singkarak, Sumatera Barat

Sayati Mandia, Netti Marusin, Putra Santoso

Abstract

A study on kidney histology of Silver Sharkminnow (Osteochilus hassletii) sampled from Maninjau and Singkarak Lakes, West Sumatra has been done from December 2012 to April 2013 at Animal Structure and Development Laboratory, Department of Biology, Andalas University, Padang. Direct observation and purposive sampling were conducted at four locations around Singkarak Lake (Sumani, Paninggahan, Sumpur, Ombilin) and one location in Maninjau Lake (Tanjung Sani). Histological samples of kidneys were isolated, fixated, dehydrated and embedded on paraffin and stained using Haematoxylin-Eosin. The results showed some histological alterations of kidney samples from all locations. Those alteration varied from cell hypertrophy, lysis, necrosis and emerging of scrape tissues. There were no significant differences on the degree of alteration among all populations. Those result indicated that both lakes had been experienced organic pollutions.
Keywords: Silver Sharkminnow (Osteochilus hasseltii C.V), Histopatology, Kidney, Singkarak Lake, Maninjau Lake.

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References

Bevelander, G dan J. Ramaley. 1988. Dasar-dasar Histologi. Penerbit Erlangga :Jakarta

Camargo, M. M. P dan C. B. R. Martinez. 2007. Histopathology of Gills, Kidney and Liver of a neutropical fish caged in an urban stream. Neotropical Ichtiology, 5(3):327-336, 2007.

Cheville N. F. 1999. Introduction to Veterinary Pathology. 2nd ed. United State of America: Iowa State University Press.

Dellman, D and Brown, E. M.. 1992. Buku Teks Histologi Veteriner II. Penerbit Universitas Indonesia. Jakarta.

Effendi, Hefni. 2000. Telaahan kualitas air. IPB: Bogor

Handy, R. D. and W. S. Penrice. 1993. The influence of high oral doses of mercuric chloride on organ toxicant concentrations and histopathology in rainbow trout, Oncorhynchus mykiss. Comparative Biochemistry and Physiology (C), 106: 717-724.

Hinton, D. E. and Lauren, D. J. 1990. Integrative Histopathological Approaches to Detecting Effects of Environmental Stressors on Fishes. In: Biological Indicators of Stress in Fish, Adams, S.M. (Ed.).American Fisheries Society, Bethesda,MD.

Kementerian Lingkungan Hidup [KLH]. 2011. Profil1 5 Danau Prioritas Nasional 2010-2014. Kementrian Lingkungan Hidup. Jakarta.

.2009. Laporan KLHS Kabupaten Agam. Kementrian Lingkungan Hidup. Jakarta.

Kottelat M., Whitten A. J., Kartikasari S. N., Wirdjoadmodjo S. 1993. Freshwater fishes of Western Indonesia and Sulawesi. Periplus. Jakarta.

Pacheco, M. & M. A. Santos. 2002. Biotransformation, genotoxic and histopathological effects of environmental contaminants in European eel (Anguilla anguilla L.). Ecotoxicology and Environmental Safety, 53: 331-347.

Pathan T. S., Shinde, S. E., Thete, P. B., and Sonawane, D. L. 2010. Histopathology of Liver and Kidney of Rasbora daniconius Exposed to paper Mill Effluent. Department of Zoology, Kalikadevi Arts, Commerce and Sciense College, Shirur (K.A.) Shirur, District Beed (M.S),India.

Price, S. A., Wilson, L. M. 1992. Clinical Concepts of Diseases Processes. Mosby Year Book, Inc.

Sawyer, C. N and McCarthy, P. L. 1978. Chemistry for Environment Engineering. Third Edition. McGraw-Hill Book Company. Tokyo. 532p.

Schwaiger, J., Wanke, R., Adam, S. Pawert, M., Honnen, W. & Triebskorn, R. 1997. The use of histopatological indicators to evaluate contaminant-related stress in fish. Journal of Aquatic Ecossystem, Stress and Recovery, 6:75-86.

Soemirat, J. (2003). Toksikologi Lingkungan .Gaja Mada University Press. Yogyakarta.

Suntoro, S. H. 1983. Metode Pewarnaan; Histologi dan Histokimia. Bhratara Karya Aksara. Jakarta.

Syandri H. 1996. Aspek reproduksi ikan bilih, Mystacoleuseus padangensis Bleeker dan Kemungkinan Pembenihannya di Danau Singkarak [Tesis]. Program Pascasarjana, Institut Pertanian Bogor. Bogor.

Syandri, H. 2008. Ancaman Terhadap Plasma Nutfah Ikan Bilih (Mystacoleuseus padangensis Blkr) dan Upaya Pelestariannya di Danau Singkarak. FakultasPerikanan dan Ilmu Kelautan. Universitas Bung Hatta. 29 hlm.

Takashima, F. and Hibya, T. 1995. An atlas of fish histology: normal and pathological features, 2nd ed. Tokyo, Kodansha.

Thophon, S., M. Kruatrachue, Upathan, E. S., Pokethitiyook, P., Sahaphong, S., Jarikhuan, S. 2003. Histopathological alterationsof white seabass, Lates calcarifer in acute and subchroniccadmium exposure. Environmental Pollution, 121: 307-320.

DOI: https://doi.org/10.25077/jbioua.2.3.%25p.2013

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